实时荧光PCR技术快速鉴别检测H5、H9、H7亚型禽流感灭活疫苗的研究

Research of Real-time Fluorescent PCR in the Rapid Differential Detection of H5、H9、H7 Subtype Avian Influenza Inactivated Vaccines

摘要:

选取H5、H9、H7亚型禽流感病毒（avian influenza virus, AIV）血凝素( hemagglutinin, HA) 基因保守序列，利用Primer Express 2.0软件设计了各自亚型的特异性引物和Taqman MGB探针，利用实时荧光RT-PCR一步法建立了H5、H9、H7亚型禽流感灭活疫苗的鉴别检测方法，该方法特异性好，重复性佳，对其他疫苗无交叉反应。结果表明实时荧光PCR方法为禽流感灭活疫苗提供了一种特异、敏感、快速和简洁的鉴别检测方法。

Abstract:

Specific primers and TaqMan MGB probes were designed with Primer Express 2.0 software according to the conserved region of the H5, H9, H7 subtype AIV hemagglutinin gene to make research of real-time fluorescent one-step PCR in the differential detection of H5,H9,H7 subtype avian influenza inactivated vaccines. The result showed that the method was specific and reproducible. No cross-reaction was discovered with other avian disease vaccines. Real-time fluorescent PCR provided a specific, sensitive, rapid and convenient method for the subtype identification of avian influenza inactivated vaccines.