Spindle-view系统在猪卵母细胞去核中的应用

Application of Spindle-view in the Enucleation Porcine of Oocytes

摘要:

应用Spindle-view对体外成熟培养36、42、44和48h的猪体外成熟卵母细胞减数分裂纺锤体进行去核操作，并与传统去核方法（McGrath-Solter去核法，挤压去核法）相比较，结果表明：①在42～48h之间利用Spindle-view得到的猪卵纺锤体影像与极体的相对位置没有明显的变化；②Spindle-view适合用于猪体外成熟卵母细胞减数分裂纺锤体的观察及去核；去核效率与其他两种方法相比差异极显著(95.5%,42.1%,74.2%,P<0.01)；③纺锤体成像是否清晰可用于猪卵母细胞的质量监控。

关键词:Spindle-view, 猪卵母细胞, 去核, 减数分裂纺锤体

Abstract:

The Spindle-view，a specialized instrument for observing spindle image，was applied to observe the meiotic spindles of vitro matured porcine oocytes at 36、42、44、48h，and enucleation from porcine, comparing to the previously methods (McGrath-Solter's method and two-step-squeezing method) in the enucleated. The results showed that:①there was no noticeable differences at vicinity of spindle images and 1st polar body among in vitro matured porcine oocytes at 40～48h under the instrument；②Spindle-view is suitable for the observation of meiotic spindles of matured oocytes and enucleation from porcine; the modified Spindle-view method for enucleation is significantly better than McGrath-Solter's method and two-step-squeezing method in the enucleated rates (95.5%,42.1%,74.2%,P<0.01)of absolutely removing nuclei matter;③the spindle images could be used to monitor the oocyte qualities.

Key words:Spindle-view， porcine oocytes，enucleation， meiotic spindle