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生物工程学报

2025年3月23日 7:15 星期日
首页 > 过刊浏览>2025年第41卷第2期 >680-692. DOI:10.13345/j.cjb.240299
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镉胁迫下丛枝菌根真菌对彩叶草生理代谢和根系损伤的影响
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国家自然科学基金(41571049,30540056);浙江省大学生创新创业训练计划项目(S202310345050)


Arbuscular mycorrhizal fungi improve physiological metabolism and ameliorate root damage of Coleus scutellarioides under cadmium stress
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    摘要:

    土壤镉污染会对观赏植物彩叶草的种植造成不利影响，当土壤受到镉污染时，彩叶草的发育会受阻，甚至可能因镉的毒性积累而死亡。本研究以金边彩叶草为实验植物，摩西斗管囊霉为供试菌，通过测定彩叶草的生理代谢和根系损伤程度，探究丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对彩叶草适应镉胁迫的影响。结果表明，镉胁迫致使彩叶草细胞内超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(ceroxidase,POD)以及过氧化氢酶(catalase,CAT)的活性、丙二醛(malondialdehyde,MDA)和脯氨酸(proline,Pro)含量上升，菌根侵染率、根系活力则受到不同程度的抑制，生长速率减慢。相同镉浓度下，接种AMF显著改善了彩叶草的各项生理指标，其中MDA含量最大降幅为42.16%，次生代谢产物迷迭香酸和花色素苷的含量最多可分别增长27.43%和25.72%，根系活力增幅高达35.35%，根系DNA损伤得到明显修复。综上所述，通过接种AMF可促进彩叶草次生代谢产物积累，缓解根系损伤，增强其对镉胁迫的耐受性。

    Abstract:

    Soil cadmium pollution can adversely affect the cultivation of the ornamental plant, Coleus scutellarioides. Upon cadmium contamination of the soil, the growth of C. scutellarioides is impeded, and it may even succumb to the toxic accumulation of cadmium. In this study, we investigated the effects of arbuscular mycorrhizal fungi (AMF) on the adaptation of C. scutellarioides to cadmium stress, by measuring the physiological metabolism and the degree of root damage of C. scutellarioides, with Aspergillus oryzae as the test fungi. The results indicated that cadmium stress increased the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and the content of malondialdehyde (MDA) and proline (Pro) within the cells of C. scutellarioides, but inhibited mycorrhizal infestation rate, root vigour and growth rate to a great degree. With the same cadmium concentration, the inoculation of AMF significantly improved the physiological indexes of C. scutellarioides. The maximum decrease of MDA content was 42.16%, and the content of secondary metabolites rosemarinic acid and anthocyanosides could be increased by up to 27.43% and 25.72%, respectively. Meanwhile, the increase of root vigour was as high as 35.35%, and the DNA damage of the root system was obviously repaired. In conclusion, the inoculation of AMF can promote the accumulation of secondary metabolites, alleviate root damage, and enhance the tolerance to cadmium stress in C. scutellarioides.

    参考文献
    [1] KUMARA SM, RANI SU, AIL G. Anticancer potential of rosmarinic acid and its improved production through biotechnological interventions and functional genomics[J]. Applied Microbiology and Biotechnology, 2018, 102(18): 7775-7793.
    [2] 刘戈宇, 柴团耀, 孙涛. 超富集植物遏蓝菜对重金属吸收、运输和累积的机制[J]. 生物工程学报, 2010, 26(5): 561-568. Liu GY, Chai TY, Sun T. Mechanisms of heavy metal absorption, transport and accumulation in the hyperaccumulator plant Draba nemerosa[J]. Chinese Journal of Biotechnology, 2010, 26(5): 561-568(in Chinese).
    [3] Peter P, Oliver H, Pepe H, Martin C, Alan P. Three species of Coleus (Lamiaceae) from the Guinean Highlands: a new species, a new combination and clarification of Coleus splendidus[J]. Kew Bulletin, 2019, 74(3): 1-14.
    [4] 李玉双, 孙丽娜, 王升厚, 孙铁珩. EDTA对4种花卉富集Cd、Pb的效应[J]. 环境科学与技术, 2007, 30(7): 16-17, 34, 116. LI YS, SUN LN, WANG SH, SUN TH. Role of EDTA in cadmium and lead accumulation by flowers[J]. Environmental Science & Technology, 2007, 30(7): 16-17, 34, 116(in Chinese).
    [5] LEVITA J, SUMIWI S, PRATIWI TI, ILHAM E, SIDIQ SP, MOEKTIWARDOYO M. Pharmacological activities of Plectranthus scutellarioides (L.) R.Br. leaves extract on cyclooxygenase and xanthine oxidase enzymes[J]. Journal of Medicinal Plants Research, 2016, 10: 261-269.
    [6] GABOROVA M, SMEJKAL K, KUBINOVA R. Abietane diterpenes of the genus Plectranthus sensu lato[J]. Molecules. 2021, 27(1): 166.
    [7] HE PP, LV XZ, WANG GY. Effects of Se and Zn supplementation on the antagonism against Pb and Cd in vegetables[J]. Environment International, 2004, 30(2): 167-172.
    [8] 王立, 汪根, 马放, 油勇强. 超积累植物与丛枝菌根真菌共生及其联合吸收积累重金属的效应[J]. 生物工程学报, 2021, 37(10): 3604-3621. WANG L, WANG G, MA F, YOU YQ. Symbiosis between hyperaccumulators and arbuscular mycorrhizal fungi and their synergistic effect on the absorption and accumulation of heavy metals: a review[J]. Chinese Journal of Biotechnology, 2021, 37(10): 3604-3621(in Chinese).
    [9] 孙红, 郑玉龙, 林炎丽, 陈超, 杨富裕. 生物炭、磷及AMF对Cd胁迫下柳枝稷生长及土壤性质的影响[J]. 草业学报, 2021, 30(12): 71-80. SUN H, ZHENG YL, LIN YL, CHEN C, YANG FY. Effects of biochar, phosphorus addition and AMF inoculation on switchgrass growth and soil properties under Cd stress[J]. Acta Prataculturae Sinica, 2021, 30(12): 71-80(in Chinese).
    [10] KHAN A, SHARIF M, ALI A, SHAH SNM, AHMAD MIAN I, WAHID F, JAN B, ADNAN M, NAWAZ S, ALI N. Potential of AM fungi in phytoremediation of heavy metals and effect on yield of wheat crop[J]. American Journal of Plant Sciences, 2014, 5(11): 1578-1586.
    [11] SALE V, SIEVERDING E, OEHL F. Growth responses of three European weeds on different AMF species during early development[J]. Plants, 2022, 11(15): 2020.
    [12] 盛萍萍, 刘润进, 李敏. 丛枝菌根观察与侵染率测定方法的比较[J]. 菌物学报, 2011, 30(4): 519-525. SHENG PP, LIU RJ, LI M. Methodological comparison of observation and colonization measurement of arbuscular mycorrhizal fungi[J]. Mycosystema, 2011, 30(4): 519-525(in Chinese).
    [13] 贾若凌. 铜胁迫对菊芋幼叶生理生化指标的影响[J]. 河南农业科学, 2012, 41(8): 154-156. JIA RL. Effects of Cu treatment on physiological and biochemical characters of erusalem artichoke (Helianthus tuberosus) leaves[J]. Journal of Henan Agricultural Sciences, 2012, 41(8): 154-156(in Chinese).
    [14] 姚虹宇, 刘亚敏, 张盛楠, 刘玉民, 周文颖, 王针针. 外源柠檬酸对铝胁迫下马尾松生理特性的影响[J]. 林业科学, 2018, 54(7): 155-164. YAO HY, LIU YM, ZHANG SN, LIU YM, ZHOU WY, WANG ZZ. Effects of exogenous citric acid on physiological characteristics of Pinus massoniana under aluminum stress[J]. Scientia Silvae Sinicae, 2018, 54(7): 155-164(in Chinese).
    [15] 吴敏, 张文辉, 周建云, 马闯, 韩文娟. 干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响[J]. 生态学报, 2014, 34(15): 4223-4233. WU M, ZHANG WH, ZHOU JY, MA C, HAN WJ. Effects of drought stress on growth, physiological and biochemical parameters in fine roots of Quercus variabilis Bl. seedlings[J]. Acta Ecologica Sinica, 2014, 34(15): 4223-4233(in Chinese).
    [16] 肖强, 郑海雷, 陈瑶, 黄伟滨, 朱珠. 盐度对互花米草生长及脯氨酸、可溶性糖和蛋白质含量的影响[J]. 生态学杂志, 2005, 24(4): 373-376. XIAO Q, ZHENG HL, CHEN Y, HUANG WB, ZHU Z. Effects of salinity on the growth and proline, soluble sugar and protein contents of Spartina alterniflora[J]. Chinese Journal of Ecology, 2005, 24(4): 373-376(in Chinese).
    [17] 刘冉, 寇亚平, 陈庆阳, 牛鹏飞, 王晓飞, 葛红, 贾瑞冬, 赵鑫, 杨树华. 蔷薇属植物花青素苷的测定及其对花瓣呈色的影响[J]. 植物遗传资源学报, 2024, 25(5): 800-812. LIU R, KOU YP, CHEN QY, NIU PF, WANG XF, GE H, JIA RD, ZHANG X, YANG SH. Determination of anthocyanin glycosides in Rosaceae plants and their effects on petal coloration[J]. Journal of Plant Genetic Resources, 2024, 25(5): 800-812(in Chinese).
    [18] 吴春太, 周珺, 姚行成, 陈先红, 王军, 林位夫. 橡胶树叶片花色素苷的提取与稳定性及其品种间含量变化研究[J]. 中南林业科技大学学报, 2017, 37(12): 15-20, 45. WU CT, ZHOU J, YAO XC, CHEN XH, WANG J, LIN WF. Study on the extraction and stability of anthocyanin from leaves of Hevea brasiliensis and its content change between three different cultivars[J]. Journal of Central South University of Forestry & Technology, 2017, 37(12): 15-20, 45(in Chinese).
    [19] Vasilik PM, Belova IN, Lazareva ME, Kononenko NV, Fedoreyeva IL. Salt tolerance assessment in Triticum aestivum and Triticum durum[J]. Frontiers in Bioscience-Landmark, 2024, 29(4): 150.
    [20] GICHNER T. DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco: cellular and acellular comet assays[J]. Mutation Research, 2003, 535(2): 187-193.
    [21] 杜军良, 杨凤, 万燕, 李静, 何志坚. 分光光度法测定3种中草药中的镉含量[J]. 江苏农业科学, 2015, 43(4): 299-301. DU JL, YANG F, WAN Y, LI J, HE ZJ. Determination of cadmium in three Chinese herbal medicines by spectrophotometry[J]. Jiangsu Agricultural Sciences, 2015, 43(4): 299-301(in Chinese).
    [22] DOWNS TM, RADFORD IJ. Arbuscular mycorrhizal fungal colonisation of Hieracium lepidulum roots in experimental and field soil inoculated media[J]. New Zealand Journal of Botany, 2005, 43(4): 843-850.
    [23] SKELDON AC, DIJK D. Weekly and seasonal variation in the circadian melatonin rhythm in humans: entrained to local clock time, social time, light exposure or sun time[J]. Journal of Pineal Research, 2021, 71(1): e12746.
    [24] BISHT A, GARG N. AMF species improve yielding potential of Cd stressed pigeonpea plants by modulating sucrose-starch metabolism, nutrients acquisition and soil microbial enzymatic activities[J]. Plant Growth Regulation, 2022, 96(3): 409-430.
    [25] Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease[J]. Toxicology, 2011, 283(2-3): 65-87.
    [26] YAN H, FILARDO F, HU XT, ZHAO XM, FU DH. Cadmium stress alters the redox reaction and hormone balance in oilseed rape (Brassica napus L.) leaves[J]. Environmental Science and Pollution Research International, 2016, 23(4): 3758-3769.
    [27] LI M, MA GS, LIAN H, SU XL, TIAN Y, HUANG WK, MEI J, JIANG XL. The effects of Trichoderma on preventing cucumber fusarium wilt and regulating cucumber physiology[J]. Journal of Integrative Agriculture, 2019, 18(3): 607-617.
    [28] Bharati L, Mayank R, Wricha T. Playing with colours: genetics and regulatory mechanisms for anthocyanin pathway in cereals[J]. Biotechnology & genetic engineering review?????????″?????????圲?丮?婢???夲?九?????????丠?堬夠????佄??奉??删?乍?删????啁?华?????么??坕????乆??奁???啒?????儬??????奎?乁??奆????慚捋琠敁爬椠慂汁?捊潉洠灁漬猠楁瑌楊潗湁??晅畁渠捎瑉椬漠湓?慙湅摄?瑁桁敓?攠湁牬楬捥桶浩敡湴瑩?潧映?灨汲慯湭瑩?杭爭潩睮瑤桵?灥牤漠浯潸瑩楤湡杴?牶桥椠穳潴扲慥捳瑳攠物楮愠??倾?偩剧??椠湲?摤楩晡晴敡爼支湩琾椠慴汨?牯桵楧穨漠獥灸桯敧牥敮?捵潳洠灴慲牥瑨浡敬湯瑳獥?潡晰??汩?瑡潴汩敯牮愺渠瑩?獳潩祧扨整慳渠?楮湴?愠捧楲摯楷捴?猬漠楰汨孯?嵯???整牨敥摴楩瑣愠獥???っ????????????????ふ??楩湥??栠極湰整獡敫?? and reactive oxygen species scavenging enzyme activity enhancement[J]. BMC Plant Biology, 2024, 24(1): 460.
    [30] 师凯丽, 王莉, 宋青媛, 王艳艳, 王兴悦, 刘智康, 蒋静静, 吴婷玉, 邱天, 金鹏, 郑永华. 甘氨酸甜菜碱在果蔬采后冷害控制中的研究进展[J]. 食品与发酵工业, 2024, 50(21): 379-387. SHI KL, WANG L, SONG QY, WANG YY, WANG XY, LIU ZK, JIANG JJ, WU TY, QIU T, JIN P, ZHENG YH. Research progress of glycine betaine in post harvest cold damage control of fruits and vegetables[J]. Food and Fermentation Industry, 2024, 50(21): 379-387(in Chinese).
    [31] AHMAD M, WARAICH EA, ZULFIQAR U, YONG JWH, ISHFAQ M, DIN KU, ULLAH a, ABBAS A, AWAN mi, MOUSSA IM, ELSHIKH MS. Thiourea improves yield and quality traits of Brassica napus L. by upregulating the antioxidant defense system under high temperature stress[J]. Scientific Reports, 2024, 14(1): 12195-12195.
    [32] DU XR, SU MX, JIAO Y, XU SX, SONG JQ, WANG HF, LI QL. A transcription factor SlNAC10 gene of Suaeda liaotungensis regulates proline synthesis and enhances salt and drought tolerance[J]. International Journal of Molecular Sciences, 2022, 23(17): 9625.
    [33] WANG XN, YANG F, ZHANG JC, REN YR, AN JP, CHANG DY, WANG XF, YOU CX. Ectopic expression of MmCYP1A1, a mouse cytochrome P450 gene, positively regulates stress tolerance in apple calli and Arabidopsis[J]. Plant Cell Reports, 2023, 42(2): 433-448.
    [34] 刘朝荣, 张柳青, 杨艳, 黄兴, 黎云祥, 权秋梅, 朱晓华. 珙桐幼苗生理生化指标对重金属铅、镉胁迫的响应[J]. 广西植物, 2021, 41(9): 1401-1410. LIU CR, ZHANG LQ, YANG Y, HUANG X, LI YX, QUAN QM, ZHU XH. Effects of lead and cadmium on physiology and biochemical indexes of Davidia involucrata seedlings[J]. Guihaia, 2021, 41(9): 1401-1410(in Chinese).
    [35] 阮仕琴, 陶刚, 娄璇, 赵兴丽. 丛枝菌根真菌生态功能及其与共生植物互作机理[J]. 中国土壤与肥料, 2022(5): 237-244. RUAN SQ, TAO G, LOU X, ZHAO XL. The ecological functions of arbuscular mycorrhizal fungi and their interaction mechanisms with the symbiotic plants[J]. Soil and Fertilizer Sciences in China, 2022(5): 237-244(in Chinese).
    [36] 朱亚琼, 黎松松, 王宁欣, 郑伟, 聂芳, 孙强, 张云玲. 根瘤菌-根系构型-丛枝菌根真菌对混播草地氮素固定与转移效率的影响[J]. 中国草地学报, 2022, 44(11): 18-31. ZHU YQ, LI SS, WANG NX, ZHENG W, NIE F, SUN Q, ZHANG YL. Effects of Rhizobium-root system-arbuscular mycorrhizal fungi features on nitrogen fixation and transfer efficiency of mixed sowing meadow[J]. Chinese Journal of Grassland, 2022, 44(11): 18-31(in Chinese).
    [37] EL RASAFI T, OUKARROUM A, HADDIOUI A, SONG H, KWON EE, BOLAN N, TACK FMG, SEBASTIAN A, PRASAD MNV, RINKLEBE J. Cadmium stress in plants: a critical review of the effects, mechanisms, and tolerance strategies[J]. Critical Reviews in Environmental Science and Technology, 2022, 52(5): 675-726.
    [38] 张志芳, 豁泽春. 镉、砷胁迫下接种丛枝菌根真菌对烤烟镉、砷累积及生理特性的影响[J]. 河南农业科学, 2022, 51(2): 47-56. ZHANG ZF, HUO ZC. Effects of arbuscular mycorrhizal fungi inoculation on cadmium, arsenic accumulation and physiological characteristics of flue-cured tobacco under cadmium and arsenic stress[J]. Journal of Henan Agricultural Sciences, 2022, 51(2): 47-56(in Chinese).
    [39] 高冰, 高鹏飞, 范卫芳, 冯郑红, 吴建慧. 镉胁迫对绢毛委陵菜-丛枝菌根真菌共生体根系结构和生理的影响[J]. 植物研究, 2022, 42(4): 647-656. GAO B, GAO PF, FAN WF, FENG ZH, WU JH. Effects of cadmium stress on the root structure and physiology of symbiont of Potentilla sericea and arbuscular mycorrhizal fungi[J]. Bulletin of Botanical Research, 2022, 42(4): 647-656(in Chinese).
    [40] IGNATENKO AA, TALANOVA VV, REPKINA NS, TITOV AF. Effect of salicylic acid on antioxidant enzymes and cold tolerance of cucumber plants[J]. Russian Journal of Plant Physiology, 2021, 68(3): 491-498.
    [41] GONG X, TIAN QD. Study on the effect mechanism of arbuscular mycorrhiza on the absorption of heavy metal elements in soil by plants[J]. IOP Conference Series: Earth and Environmental Science, 2019, 267(5): 52-64.
    [42] Karzan Q, Karokh K, Avin S, Haider H, Safin H,Zhilia H, Rayan A, Banw A, Zahra A. Aeromonas sobria as a potential candidate for bioremediation of heavy metal from contaminated environments[J]. Scientific Reports, 2022, 12(1): 21235-21235.
    [43] 苗志加, 孟祥源, 李书缘, 马超, 李晴, 安贺銮, 赵鑫, 赵志瑞. 丛枝菌根真菌修复重金属污染土壤及增强植物耐性研究进展[J]. 农业环境科学学报, 2023, 42(2): 252-262. MIAO ZJ, MENG XY, LI SY, MA C, LI Q, AN HL, ZHAO X, ZHAO ZR. Research progress on arbuscular mycorrhizal fungi (AMF) in remediation of heavy metal contaminated soil and enhancement of plant tolerance[J]. Journal of Agro-Environment Science, 2023, 42(2): 252-262(in Chinese).
    [44] ZHOU S, YANG JH, YAN SW, YAN YT, ZHANG LY, YE WL. Research progress on the effects of rhizosphere organic acids on the chemical behavior and bioavailability of heavy metals in soil[J]. Journal of Biological Chemistry, 2022, 39(3): 103-106.
    [45] TIAN LX, LI J. The effects of exogenous ABA applied to maize (Zea mays L.) roots on plant responses to chilling stress[J]. Acta Physiologiae Plantarum, 2018, 40(4): 77.
    [46] SONG P, ZHUO QM, DING Y F. Identification of anthocyanin in Euonymus europaea leaves and study on their stability[J]. Chinese Wild Plant Resources, 2020, 39(3): 11-18, 32.
    [47] 杜红, 李玉鹏, 程文, 肖荣英, 胡鹏. 丛枝菌根真菌改善镉胁迫下植物根系和土壤微环境的效应[J]. 浙江农业学报, 2022, 34(5): 1039-1048. DU H, LI YP, CHENG W, XIAO RY, HU P. Effects of arbuscular mycorrhizal fungi on plant roots and soil microenvironment under cadmium stress[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1039-1048(in Chinese).
    [48] ZHAO DK, WANG HP, CHEN SY, YU DQ, REITER RJ. Phytomelatonin: an emerging regulator of plant biotic stress resistance[J]. Trends in Plant Science, 2021, 26(1): 70-82.
    [49] 宋婕. 镉胁迫对拟南芥幼苗DNA损伤及修复的影响[D]. 沈阳: 辽宁大学硕士学位论文, 2017. SONG J. Effect of Cd-induced DNA damage and DNA repair system on Arabidopsis seedlings[D].Shenyang: Master’s Thesis of Liaoning University, 2017(in Chinese).
    [50] 文钟灵, 杨旻恺, 陈星雨, 郝晨宇, 任然, 储淑娟, 韩洪苇, 林红燕, 陆桂华, 戚金亮, 杨永华. 酸铝胁迫土壤中耐铝大豆根际不同部位细菌群落结构、功能及其对促生菌富集作用的研究[J]. 遗传, 202
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侯亚男,江帆,周舒扬,陈丁尹,朱怡杰,缪怡宁,岑锎,王怡芳,吴敏,刘鹏. 镉胁迫下丛枝菌根真菌对彩叶草生理代谢和根系损伤的影响[J]. 生物工程学报, 2025, 41(2): 680-692

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