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2025年3月23日 3:35 星期日
首页 > 过刊浏览>2025年第41卷第2期 >809-824. DOI:10.13345/j.cjb.240498
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云南栘[木衣]TCP基因家族全基因组鉴定与表达分析
作者:
基金项目:

云南省基础研究计划(202401AS070042);国家自然科学基金(32060350);西南林业大学林业创新项目(LXXK-2023Z01);云南省“万人计划”青年拔尖人才项目(YNWR-ONBJ-2020-230)


Genome-wide identification and expression analysis of TCP gene family in Docynia delavayi (Franch.) Schneid.
Author:
  • ZHANG Baoyue

    ZHANG Baoyue

    Key Laboratory of Ministry of Education for Conservation and Utilization of Mountain Forest Resources in Southwest China, Southwest Forestry University, Kunming 650224, Yunnan, China;Key Laboratory for Forest Genetic and Tree Improvement &Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, Yunnan, China
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  • LIU Guoping

    LIU Guoping

    Key Laboratory of Ministry of Education for Conservation and Utilization of Mountain Forest Resources in Southwest China, Southwest Forestry University, Kunming 650224, Yunnan, China;Key Laboratory for Forest Genetic and Tree Improvement &Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, Yunnan, China
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  • TIAN Jinhong

    TIAN Jinhong

    Key Laboratory of Ministry of Education for Conservation and Utilization of Mountain Forest Resources in Southwest China, Southwest Forestry University, Kunming 650224, Yunnan, China;Key Laboratory for Forest Genetic and Tree Improvement &Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, Yunnan, China
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  • WANG Dawei

    WANG Dawei

    Key Laboratory of Ministry of Education for Conservation and Utilization of Mountain Forest Resources in Southwest China, Southwest Forestry University, Kunming 650224, Yunnan, China;Key Laboratory for Forest Genetic and Tree Improvement &Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, Yunnan, China
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    摘要:

    云南栘[木衣](Docynia delavayi (Franch.) Schneid.)作为一种经济林果树，具有较高药用和食用价值。TCP (Teosinte branched1/Cycloidea/Proliferating cell factor)基因家族在植物整个生长发育过程中发挥着重要的作用。为探讨云南栘[木衣]TCP基因家族在其生长发育过程中的作用，本研究利用生物信息学方法对云南栘[木衣]TCP基因家族进行了全基因组鉴定，并分析了其在种子萌发和果实发育不同时期的表达水平。结果显示，云南栘[木衣]共有18个DdeTCP基因，不均匀地分布在11条染色体上。系统进化分析结果显示，DdeTCP在Class Ⅰ中有3个成员，Class Ⅱ类有15个成员，表明DdeTCP家族成员间出现了功能分化。基因表达模式分析显示，AtTCP14同源基因DdeTCP11在种子萌发前期具有较高的表达水平，表明其可能在种子萌发时期发挥重要作用。此外，聚类在Class Ⅰ中的DdeTCP16在果实成熟期表达量较高，推测其可能与果实成熟有关。本研究为进一步探索DdeTCP基因家族在云南栘[木衣]生长发育过程中的功能奠定了基础。

    Abstract:

    Docynia delavayi (Franch.) Schneid. is an economic fruit plant with high medicinal and edible values. The TCP gene family plays a vital role in plant growth and development. To explore the function of the TCP gene family in the growth and development of D. delavayi. In this study, the TCP gene family (DdeTCP) members were identified from the D. delavayi genome and their expression levels at different stages of seed germination and fruit development were analyzed. The results showed that a total of 18 DdeTCP genes were identified from the D. delavayi genome, with uneven location on 11 chromosomes. The phylogenetic tree showed that the 18 DdeTCPs could be classified into class I (3) and class II (15), suggesting that functional differentiation occurred among the DdeTCP family members. DdeTCP11 highly homologous to AtTCP14 was highly expressed in the early stage of seed germination, which suggested that this gene played a key role in seed germination. In addition, DdeTCP16 in class I had a high expression level during the fruit ripening stage, which indicated that it might be related to fruit ripening. The findings lay a foundation for probing into the roles of the DdeTCP gene family in the growth and development of D. delavayi.

    参考文献
    [1] ZHAO XP, SHU GW, CHEN LY, MI X, MEI ZN, DENG XK. A flavonoid component from Docynia delavayi (Franch.) Schneid. represses transplanted H22 hepatoma growth and exhibits low toxic effect on tumor-bearing mice[J]. Food and Chemical Toxicology, 2012, 50(9): 3166-3173.
    [2] XIA X, CHEN C, YANG L, WANG YC, DUAN AN, WANG DW. Analysis of metabolites in young and mature Docynia delavayi (Franch.) Schneid. leaves using UPLC-ESI-MS/MS[J]. PeerJ, 2022, 10: e12844.
    [3] 刘刚, 吴京, 朱明君, 张晓喻, 张宏. 云南栘[木衣]叶提取物片段抗氧化活性及成分分析[J]. 西南师范大学学报(自然科学版), 2014, 39(9): 73-81. LIU G, WU J, ZHU MJ, ZHANG XY, ZHANG H. On evaluation of antioxidant activities and screening of potential antioxidant compounds from Docynia delavayi (Franch.) Schneid. leaves[J]. Journal of Southwest China Normal University (Natural Science Edition), 2014, 39(9): 73-81(in Chinese).
    [4] 李学玲, 张建强, 郑琰, 陈云兰, 黄竹珺. 大果栘依总黄酮的提取与抗氧化性研究[J]. 云南化工, 2018, 45(12): 58-62. LI XL, ZHANG JQ, ZHENG Y, CHEN YL, HUANG ZJ. Study on extraction, identification and antioxidation activity of flavonoids from Docynia delavayi (Franch.) Schneid.[J]. Yunnan Chemical Technology, 2018, 45(12): 58-62(in Chinese).
    [5] 王安娜, 彭小伟, 阚欢, 王大玮, 胡祥, 刘云. 云南栘依黄酮提取及其抗氧化、降血糖活性研究[J]. 食品工业科技, 2023, 44(2): 232-240. WANG AN, PENG XW, KAN H, WANG DW, HU X, LIU Y. Extraction of flavonoids from Docynia delavayi and their antioxidant and hypoglycemic activities[J]. Science and Technology of Food Industry, 2023, 44(2): 232-240(in Chinese).
    [6] 王溪唯, 陈璨, 王大玮. 云南栘[木衣]MADS-box基因家族鉴定与表达分析[J]. 生物工程学报, 2023, 39(7): 2897-2913. WANG XW, CHEN C, WANG DW. Identification and expression analysis of MADS-box gene family in Docynia delavayi (Franch.) Schneid.[J]. Chinese Journal of Biotechnology, 2023, 39(7): 2897-2913(in Chinese).
    [7] 田金红, 亚华金, 王玉昌, 杨利华, 王大玮. 云南栘[木衣]R2R3-MYB转录因子鉴定与表达分析[J]. 中南林业科技大学学报, 2023, 43(4): 144-155. TIAN JH, YA HJ, WANG YC, YANG LH, WANG DW. Identification and expression analysis of R2R3-MYB transcription factors in Docynia delavayi[J]. Journal of Central South University of Forestry & Technology, 2023, 43(4): 144-155(in Chinese).
    [8] 亚华金, 田金红, 沈莲文, 王大玮. 云南栘[木衣]bHLH转录因子家族鉴定与分析[J]. 西南农业学报, 2024, 37(9): 2003-2011. YA HJ, TIAN JH, SHEN LW, WANG DW. Identification and analysis of bHLH transcription factors family in Docynia delavayi[J]. Southwest China Journal of Agricultural Sciences, 2024, 37(9): 2003-2011(in Chinese).
    [9] 徐鎏, 刘宇, 杨林, 田金红, 李婧婷, 马海涛, 李恩良, 王大玮. 鲜食型云南栘[木衣]优树选择[J]. 植物遗传资源学报, 2023, 24(3): 903-910. XU L, LIU Y, YANG L, TIAN JH, LI JT, MA HT, LI EL, WANG DW. Plus tree selection of fresh Docynia delavayi (Franch.) Schneid.[J]. Journal of Plant Genetic Resources, 2023, 24(3): 903-910(in Chinese).
    [10] 张新洛, 彭劲谕, 王玉昌, 王大玮. 云南栘(木衣)总DNA提取方法比较及SSR反应体系优化[J]. 云南农业大学学报(自然科学), 2022, 37(6): 1064-1070. ZHANG XL, PENG JY, WANG YC, WANG DW. Comparison of extraction methods of total DNA and optimization of SSR reaction systems from Docynia delavayi[J]. Journal of Yunnan Agricultural University (Natural Science), 2022, 37(6): 1064-1070(in Chinese).
    [11] 慈晓彤, 石辰, 王大玮, 沈莲文, 何承忠, 蔡年辉, 段安安. 云南栘[木衣]叶片总RNA提取方法的比较与改进[J]. 西北林学院学报, 2020, 35(3): 95-99. CI XT, SHI C, WANG DW, SHEN LW, HE CZ, CAI NH, DUAN AA. Comparison and improvement of total RNA extraction methods from Docynia delavayi leaves[J]. Journal of Northwest Forestry University, 2020, 35(3): 95-99(in Chinese).
    [12] 陈璨, 石辰, 王大玮, 彭劲谕, 段安安. 云南栘(木衣)SRAP-PCR反应体系的建立及引物筛选[J]. 云南农业大学学报(自然科学), 2021, 36(6): 1037-1043. CHEN C, SHI C, WANG DW, PENG JY, DUAN AA. Establishment of SRAP-PCR reaction system on Docynia delavayi (Franch.) Schneid. and selection of primers[J]. Journal of Yunnan Agricultural University (Natural Science), 2021, 36(6): 1037-1043(in Chinese).
    [13] 刘宇, 朱泽莉, 彭劲谕, 王大玮, 段安安. 云南栘[木衣]ISSR-PCR反应体系的建立及引物筛选[J]. 分子植物育种, 2023, 21(21): 7097-7104. LIU Y, ZHU ZL, PENG JY, WANG DW, DUAN AA. Establishment of ISSR-PCR reaction system of Docynia delavayi (Franch.) Schneid. and primers screening[J]. Molecular Plant Breeding, 2023, 21(21): 7097-7104(in Chinese).
    [14] LI LX, OU WL, WANG YC, PENG JY, WANG DW, XU S. Comparison of genetic diversity between ancient and common populations of Docynia delavayi (Franch.) Schneid.[J]. Gene, 2022, 829: 146498.
    [15] PENG JY, SHI C, WANG DW, LI SZ, ZHAO XL, DUAN AN, CAI NH, HE CZ. Genetic diversity and population structure of the medicinal plant Docynia delavayi (Franch.) Schneid. revealed by transcriptome-based SSR markers[J]. Journal of Applied Research on Medicinal and Aromatic Plants, 2021, 21: 100294.
    [16] NICOLAS M, CUBAS P. TCP factors: new kids on the signaling block[J]. Current Opinion in Plant Biology, 2016, 33: 33-41.
    [17] PECHER P, MORO G, CANALE MC, CAPDEVIELLE S, SINGH A, MacLEAN A, SUGIO A, KUO CH, LOPES JRS, HOGENHOUT SA. Phytoplasma SAP11 effector destabilization of TCP transcription factors differentially impact development and defence of Arabidopsis versus maize[J]. PLoS Pathogens, 2019, 15(9): e1008035.
    [18] 刘璇, 陈虞超, 郭生虎, 钟楠, 石磊, 甘晓燕. 芦竹TCP家族的基因鉴定与盐胁迫下的表达分析[J]. 草业科学, 2024, 41(10): 2316-2329. LIU X, CHEN YC, GUO SH, ZHONG N, SHI L, GAN XY. Gene identification and expression analysis of TCP family of Arundo donax L. under salt stress. Pratacultural Science, 2024, 41(10): 2316-2329(in Chinese).
    [19] RESENTINI F, FELIPO-BENAVENT A, COLOMBO L, BLÁZQUEZ MA, ALABADÍ D, MASIERO S. TCP14 and TCP15 mediate the promotion of seed germination by gibberellins in Arabidopsis thaliana[J]. Molecular Plant, 2015, 8(3): 482-485.
    [20] TAKEDA T, AMANO K, OHTO MA, NAKAMURA K, SATO S, KATO T, TABATA S, UEGUCHI C. RNA interference of the Arabidopsis putative transcription factor TCP16 gene results in abortion of early pollen development[J]. Plant Molecular Biology, 2006, 61(1/2): 165-177.
    [21] DANISMAN S, van der WAL F, DHONDT S, WAITES R, de FOLTER S, BIMBO A, van DIJK ADJ, MUINO JM, CUTRI L, DORNELAS MC, ANGENENT GC, IMMINK RGH. Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically[J]. Plant Physiology, 2012, 159(4): 1511-1523.
    [22] 周延培, 张雅剑, 伊华林. 柑橘TCP家族生物信息学及表达谱分析[J]. 果树学报, 2016, 33(5): 513-522. ZHOU YP, ZHANG YJ, YI HL. Bioinformatics identification and expression analysis of the Citrus TCP gene family[J]. Journal of Fruit Science, 2016, 33(5): 513-522(in Chinese).
    [23] XIE YG, MA YY, BI PP, WEI W, LIU J, HU Y, GOU YJ, ZHU D, WEN YQ, FENG JY. Transcription factor FvTCP9 promotes strawberry fruit ripening by regulating the biosynthesis of abscisic acid and anthocyanins[J]. Plant Physiology and Biochemistry, 2020, 146: 374-383.
    [24] 杜洋, 张智韦, 陆爽, 刘雪, 李大勇, 徐朝阳, 王晨晨, 张彬, 许立新. 菊苣TCP基因家族鉴定与分析[J]. 草地学报, 2024: 1-17. DU Y, ZHANG ZW, LU S, LIU X, LI DY, XU ZY, WANG CC, ZHANG B, XU LX. Chicory TCP gene family identification and analysis[J]. Acta Agrestia Sinica, 2024: 1-17(in Chinese).
    [25] ZHAO Y, SU XQ, WANG XY, WANG MN, CHI XJ, AAMIR MANZOOR M, LI GH, CAI YP. Comparative genomic analysis of TCP genes in six Rosaceae species and expression pattern analysis in Pyrus bretschneideri[J]. Frontiers in Genetics, 2021, 12: 669959.
    [26] SCHOMMER C, PALATNIK JF, AGGARWAL P, CHÉTELAT A, CUBAS P, FARMER EE, NATH U, WEIGEL D. Control of jasmonate biosynthesis and senescence by miR319 targets[J]. PLoS Biology, 2008, 6(9): e230.
    [27] 周棋赢, 韩月华, 祝悦, 陈赛, 李先文, 彭波, 袁红雨. 茶树TCP家族的全基因组鉴定及其表达分析[J]. 园艺学报, 2019, 46(10): 2021-2036. ZHOU QY, HAN YH, ZHU Y, CHEN S, LI XW, PENG B, YUAN HY. Genome-wide identification, classification and expression analysis of TCP gene family in tea plant[J]. Acta Horticulturae Sinica, 2019, 46(10): 2021-2036(in Chinese).
    [28] CHEN CJ, CHEN H, ZHANG Y, THOMAS HR, FRANK MH, HE YH, XIA R. TBtools: an integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant, 2020, 13(8): 1194-1202.
    [29] MINH BQ, SCHMIDT HA, CHERNOMOR O, SCHREMPF D, WOODHAMS MD, von HAESELER A, LANFEAR R. IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era[J]. Molecular Biology and Evolution, 2020, 37(5): 1530-1534.
    [30] WANG YC, SONG YY, WANG DW. Transcriptomic and metabolomic analyses providing insights into the coloring mechanism of Docynia delavayi[J]. Foods, 2022, 11(18): 2899.
    [31] TATEMATSU K, NAKABAYASHI K, KAMIYA Y, NAMBARA E. Transcription factor AtTCP14 regulates embryonic growth potential during seed germination in Arabidopsis thaliana[J]. The Plant Journal, 2008, 53(1): 42-52.
    [32] KIEFFER M, MASTER V, WAITES R, DAVIES B. TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis[J]. The Plant Journal, 2011, 68(1): 147-158.
    [33] 张士刚. 苹果TCP转录因子家族生物信息学分析[J]. 山东农业科学, 2014, 46(5): 12-17. ZHANG SG. Bioinformatics analysis of TCP transcription factor family in apple[J]. Shandong Agricultural Sciences, 2014, 46(5): 12-17(in Chinese).
    [34] LENG XP, WEI HR, XU XZ, GHUGE SA, JIA DJ, LIU GS, WANG YZ, YUAN YB. Genome-wide identification and transcript analysis of TCP transcription factors in grapevine[J]. BMC Genomics, 2019, 20(1): 786.
    [35] 谭政委, 郭水柱, 苏小雨, 孙瑶, 余永亮, 李磊, 张利超, 许兰杰, 鲁丹丹, 安素妨, 李春明, 梁慧珍, 王子君. 全基因组水平金银花TCP基因家族的鉴定及表达模式分析[J]. 中草药, 2024, 55(5): 1665-1676. TAN ZW, GUO SZ, SU XY, SUN Y, YU YL, LI L, ZHANG LC, XU LJ, LU DD, AN SF, LI CM, LlANG HZ, WANG ZJ. Genome-wide analysis of TCP gene family and their expression pattern analysis in Lonicera japonica[J]. Chinese Traditional and Herbal Drugs, 2024, 55(5): 1665-1676(in Chinese).
    [36] 阮城城, 胡福初, 罗志文, 王祥和, 郭利军, 李玉静, 范鸿雁, 韩冰, 陈哲，张治礼. 菠萝TCP基因家族的鉴定及成花诱导阶段的表达谱分析[J]. 果树学报, 2020, 37(11): 1623-1635. RUAN CC, HU FC, LUO ZW, WANG XH, GUO LJ, LI YJ, FAN HY, HAN B, CHEN Z, ZHANG ZL. Genome-wide identification of pineapple TCP gene family and analysis of expression profile in flower induction stage[J]. Journal of Fruit Science, 2020, 37(11): 1623-1635(in Chinese).
    [37] YAO X, MA H, WANG J, ZHANG DB. Genome-wide comparative analysis and expression pattern of TCP gene families in Arabidopsis thaliana and Oryza sativa[J]. Journal of Integrative Plant Biology, 2007, 49(6): 885-897.
    [38] SHANG XW, HAN ZL, ZHANG DY, WANG Y, QIN H, ZOU ZW, ZHOU L, ZHU XJ, FANG WP, MA YC. Genome-wide analysis of the TCP gene family and their expression pattern analysis in tea plant (Camellia sinensis)[J]. Frontiers in Plant Science, 2022, 13: 840350.
    [39] 张志强, 卢世雄, 马宗桓, 周琪, 何红红, 陈佰鸿, 毛娟. 草莓TCP转录因子家族生物信息学鉴定及基因表达分析[J]. 西北植物学报, 2020, 40(12): 2031-2043. ZHANG ZQ, LU SX, MA ZH, ZHOU Q, HE HH, CHEN BH, MAO J. Bioinformatics identification and expression analysis of TCP transcription factor family in strawberry[J]. Acta Botanica Boreali-Occidentalia Sinica, 2020, 40(12): 2031-2043(in Chinese).
    [40] 齐香玉, 李新茹, 陈双双, 冯景, 陈慧杰, 金玉妍, 苗艳华, 邓衍明. 茉莉花TCP基因家族全基因组鉴定及其表达分析[J]. 华北农学报, 2024, 39(1): 63-71. QI XY, LI XR, CHEN SS, FENG J, CHEN HJ, JlN YY, MlAO YH, DENG YM. Genome-wide identification of TCP gene family in Jasminum sambac and expression analysis involved in flower development and pollen-pistil interaction[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 63-71(in Chinese).
    [41] ZHOU M, LI DY, LI ZG, HU Q, YANG CH, ZHU LH, LUO H. Constitutive expression of a miR319 gene alters plant development and enhances salt and drought tolerance in transgenic creeping bentgrass[J]. Plant Physiology, 2013, 161(3): 1375-1391.
    [42] FRANCIS A, DHAKA N, BAKSHI M, JUNG KH, SHARMA MK, SHARMA R. Comparative phylogenomic analysis provides insights into TCP gene functions in Sorghum[J]. Scientific Reports, 2016, 6: 38488.
    [43] CANNON SB, MITRA A, BAUMGARTEN A, YOUNG ND, MAY G. The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana[J]. BMC Plant Biology, 2004, 4: 10.
    [44] ZOU QC, DONG Q, TIAN DQ, MAO LH, CAO XR, ZHU KY. Genome-wide analysis of TCP transcription factors and their expression pattern analysis of rose plants (Rosa chinensis)[J]. Current Issues in Molecular Biology, 2023, 45(8): 6352-6364.
    [45] TIAN JH, SHU JX, XU L, YA HJ, YANG L, LI SG, WANG DW. Genome-wide identification of R2R3-MYB transcription factor family in Docynia delavayi (Franch.) Schneid. and its expression analysis during the fruit development[J]. Food Bioscience, 2023, 54: 102878.
    [46] GASTALDI V, NICOLAS M, MUÑOZ-GASCA A, CUBAS P, GONZALEZ DH, LUCERO L. Class I TCP transcription factors TCP14 and TCP15 promote axillary branching in Arabidopsis by counteracting the action of Class II TCP BRANCHED1[J]. New Phytologist, 2024, 243(5): 1810-1822.
    [47] PARAPUNOVA V, BUSSCHER M, BUSSCHER- LANGE J, LAMMERS M, KARLOVA R, BOVY AG, ANGENENT GC, de MAAGD RA. Identification, cloning and characterization of the tomato TCP transcription factor family[J]. BMC Plant Biology, 2014, 14: 157.
    [48] DANISMAN S. TCP transcription factors at the interface between environmental challenges and the plant’s growth responses[J]. Frontiers in Plant Science, 2016, 7: 1930.
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张宝月,刘国平,田金红,王大玮. 云南栘[木衣]TCP基因家族全基因组鉴定与表达分析[J]. 生物工程学报, 2025, 41(2): 809-824

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  • 收稿日期:2024-06-17
  • 最后修改日期:2024-09-11
  • 在线发布日期: 2025-02-11
  • 出版日期: 2025-02-25
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