格尔德霉素生物合成的调控基因

Regulatory Genes of Geldanamycin Biosynthesis

摘要:

从吸水链霉菌17997中克隆了格尔德霉素(Geldanamycin, Gdm)生物合成酶基因簇, 通过生物信息学分析发现两个LAL(Large ATP-binding regulators of the LuxR family)家族的调控基因gdmRI和gdmRII, 基因阻断和基因回复实验证实这两个基因产物都正调控Gdm的生物合成。

Abstract:

Two LAL family regulatory genes, gdmRI and gdmRII, were identified in the geldanamycin biosynthetic gene cluster of Streptomyces hygroscopicus 17997. Disruption of the two regulatory genes resulted in absolute elimination of geldanamycin biosynthesis. The complementation experiments using a single wild-type gene could restore geldanamycin production. These results indicated that both gdmRI and gdmRII were positive regulatory genes of the geldanamycin biosynthesis.