薯蔓多糖的分离纯化和性质鉴定

Isolation and Identification of Polysaccharide from Sweet Potato Vines

摘要:

以我国资源量巨大的薯蔓为原料, 采用中试设备, 利用水提醇沉法提取多糖。研究了活性炭脱色工艺, 经初步纯化获得薯蔓多糖(PSPV), 并研究了其理化性质。DEAE-纤维素柱对来自于二个季节的PSPV进行分离、纯化, 分别得到PSPVⅠ和PSPVⅡ、PSPVⅢ三个多糖组分, 高效凝胶过滤色谱法测定三个组分的分子量分别为6.278×104 D, 3.801×104 D和1.418×104 D, 气相色谱结合标样测定单糖组成。研究结果为充分高效利用薯蔓资源提供了理论依据。

Abstract:

China is the largest country of cultivating sweet potato in the world. The resources of sweet potato vines (SPV) are huge. To exploit the rich resources, SPV of XuShu 18 was selected as raw materials since it is cultivated in China widely. Polysaccharide from SPV was extracted by pilot instrument and precipitated by ethanol. The decolouring technology was studied. By primary purification, we obtained the polysaccharide from SPV (PSPV) and studied its physical and chemical characters. Using DEAE-cellulose column and NaCl solution as eluting reagent, three kinds of pure polysaccharide, named PSPVⅠ, PSPVⅡ and PSPVⅢ respectively, were obtained. Then the molecular weight distribution and sugar compositions were studied using High Performance Gel Filtration Chromatography and GC. The followings are the results. PSPVⅠ was separated from PSPV which was extracted from SPV harvested in the middle of June. Its M.W. is 6.278×104 D and is mainly composed of Xylose, Mannose and Glucose. PSPVⅡ and PSPVⅢ were separated from PSPV which was extracted from SPV harvested in the middle of October. Their M.W. are 3.801×104 D and 1.418×104 D respectively. PSPVⅡ is mainly composed of Mannose and Galactan. PSPVⅢ is mainly composed of Glucose, Xylose and Rhamnose. The results provide theoretical basis for the utilization of SPV.