汽爆葛根直接固态发酵乙醇联产葛根黄酮

Production of Ethanol and Isoflavones from Steam-pretreated Radix Puerariae by Solid State Fermentation

摘要:

针对葛根原料富含纤维和黄酮等特点, 选用低压汽爆处理代替糊化直接固态同步糖化发酵乙醇, 而后提取发酵剩余物的葛根黄酮。实验结果表明鲜葛根在0.8 MPa压力下维持3.5 min汽爆处理后, 直接加入糖化酶(65 u/g)、纤维素酶(1.5 u/g), 0.1%(NH4)2SO4、0.1%KH2PO4和活化后的酵母, 35~37oC下, 固态同步糖化发酵60 h, 100 g干葛根可生产的乙醇与葛根黄酮分别为27.47 g、4.43 g, 淀粉利用率达到95%。该方法实现了葛根分层多级转化清洁利用, 为非粮食类淀粉资源发酵乙醇提供了一条新途径。

Abstract:

The gelatinization process of the starch is replaced by unpolluted steam-pretreatment on the base of the Radix Puerariae rich in fiber and isoflavones. The production of ethanol and isoflavones by simultaneous saccharification and solid state fermentation (SSF) of steam-pretreatment Radix Puerariae is presented. The optimal technological conditions were obtained: Radix Puerariae being steam-pretreated at a saturated vapor pressure of 0.8 MPa for 3.5 min, glucoamylase(65 u/g), cellulase(1.5 u/g), 0.1%(NH4)2SO4, 0.1%KH2PO4 and activated yeasts being added in, and fermentation at 35~37oC for 60 h. Under these conditions, the yield of ethanol and isoflavones from 100 g Radix Pureriae (dry basis) were 27.47 g and 4.43 g, respectively, the starch utilization rate was 95%. In comparison with the traditional fermentation technology, the simultaneous saccharification and SSF of steam-pretreatment Radix Puerariae is clean and energy-saving. It provides new way of the production of ethanol from the non-food starch material, and worthwhile to be explored and implemented in industry.