trxS基因对大麦发芽籽粒中蛋白质降解的影响

Effects of trxS gene on protein degradation in germinating barley seeds

摘要:

对转trxS基因大麦籽粒发芽过程中蛋白酶活性、不同蛋白组分含量和贮藏蛋白SDS-PAGE图谱的变化进行了研究。结果表明：与对照相比，转基因籽粒中的蛋白酶活性提高；清蛋白、球蛋白、醇溶蛋白和谷蛋白含量低于对照。SDS-PAGE图谱也表明，转基因籽粒中贮藏蛋白降解快于对照。

Abstract:

We assessed the effects of trxS gene on changes of proteinase activity, contents of different protein fractions and SDS-PAGE profiles in germinating seeds of contrasting transgenic and nontransgenic barley variety. Proteinase activity was enhanced by 70.28% in transgenic than nontransgenic barley seeds, whereas contents of albumin, globulin, hordein and glutelin in transgenic seeds were 3.68%, 23.52%, 31.37%, and 21.04%, lower than those in nontransgenic seeds. Degradation rates of hordein and glutelin in transgenic seeds were faster than those in nontransgenic seedlings as indicated by the SDS-PAGE profiles. Our data imply that the transformation of trxS gene could promote the degradation of protein, providing theoretic basis for the use of trxS gene and barley quality breeding.