虾红素对小鼠肌肉组织和骨骼肌细胞中能量代谢相关基因mRNA表达的影响

Role of astacene in mice skeletal muscle and muscle cell mRNA expression of energy metabolism-related genes

摘要:

本试验用高、低浓度虾红素日粮饲喂昆白系小鼠和处理原代培养小鼠骨骼肌细胞, 提取总RNA, 检测各时段UCP3、LXRa基因mRNA表达量, 探讨虾红素对小鼠个体发育、肌肉能量代谢相关基因表达变化规律的影响。结果表明: 高浓度组与对照组相比, 小鼠体重增长明显减慢, 肌肉组织第10天、30天以及骨骼肌细胞作用24 h时UCP3 mRNA表达量均显著下降(P<0.05), LXRa基因mRNA表达量均显著上升(P<0.05), 72 h达到极显著水平(P<0.01)。低浓度组与对照组相比, 肌肉组织中UCP3、LXRa基因mRNA表达差异均不显著(P>0.05); 虾红素作用骨骼肌细胞24 h UCP3基因mRNA表达量显著下降(P<0.05), LXRa基因mRNA表达量显著上升(P<0.05)。结果提示虾红素对小鼠肌肉的能量利用有一定的调控作用。

Abstract:

In order to examine the role of astacene on mice body development and the expression of energy metabolism related genes in mice, we treated mice (Kunming white) and primary culture of mouse muscle cells with astacene of higher and lower concentration. Then the total mRNA was extracted from the muscle tissue and cells respectively, and the mRNA levels of UCP3 and LXRα were detected by RT-PCR in all the samples. Compared with the control group, the body weight of mice in high concentrations of astacene group grown slowly, and the expressions of UCP3 genes decreased significantly in muscle tissue of the 10th day and the 30th day as well as the cells of treated for 24 h (P<0.05). The expression of LXRα gene increased significantly in all samples (P<0.05) and reached its peak at 72 h (P<0.01). With the treatment of lower concentration of astacene, the expressions of UCP3 and LXRα gene mRNA in muscle tissue did not alter much, but in muscle cells treated for 24 h, the mRNA level of UCP3 gene decreased significantly (P<0.05), and LXRα gene increased significantly (P<0.05). The results suggest that astacene has a role in regulating the energy use in mice muscle.