PiA水稻悬浮细胞系的建立

Rapid establishment of suspension cell lines in japonica rice

摘要:

本研究以抗稻瘟病的粳稻PiA开花后12~15 d的幼胚为材料, 将诱导10~15 d的愈伤不经固体培养基继代, 直接转到AA液体培养基上, 在较短时间内成功建立起了优良的水稻悬浮细胞系; 并测定了该悬浮细胞系不同时期的生长特性和分化情况, 结果表明悬浮培养适宜的继代天数是7~10 d; 培养30~120 d的细胞分化能力和植株再生能力较好, 细胞分化率和成苗率分别为57.1%和20%, 为进一步利用悬浮细胞进行遗传转化和原生质体分离奠定了基础。

Abstract:

With Jingkang No.5 (PiA), calli of the PiA induced for 10?15 days were transferred into amino acid liquid culture medium, to establish excellent rice suspension cell lines successfully in a relative short time. The growth characteristics and differentiation conditions of suspension cells were measured at different phases. Results revealed that the optimal subculture time was 7?10 days, and the cells cultured for 30?120 days had the best differentiation ability (57.1%) and regeneration ability (20%). This study is promising in further using the suspension cell for genetic transformation and protoplasm isolation.