类人胶原蛋白-透明质酸血管支架的性能及生物相容性

Characterization and biocompatibility of human-like collagen-hyaluronic acid scaffold for blood vessel

摘要:

将类人胶原蛋白与透明质酸按不同比例复合, 控制透明质酸的终浓度(W/V)分别为0、0.01%、0.05%、0.1%, 用京尼平交联, 采用真空冷冻干燥方法构建出血管支架材料。通过扫描电镜、XPS分析、拉力测试、压力爆破实验、细胞毒性实验、血管支架细胞种植实验及小鼠皮下植入等方法对其表面超微结构、表面元素组成、力学性能、细胞毒性等级、细胞相容性、组织相容性进行了研究。结果表明: 当透明质酸的含量为0.05%时, 类人胶原蛋白-透明质酸支架的孔径比较均匀, 孔隙率达94.38%, 应力为(1000.8 ± 7.9) kPa, 爆破压力为(1058.6 ± 8.2) kPa, 细胞毒性实验合格, 同时具有良好的细胞相容性、组织相容性及降解性能。

Abstract:

Human-like collagen (HLC) was cross-linked with hyaluronic acid by genipin in different ratio. The concentrations of hyaluronic acid in the mixture were 0, 0.01%, 0.05% and 0.1%. The blood vessel tubular grafts were then fabricated by freeze-drying. Microstructure, element composite, mechanical properties, cytotoxicity grade, and biocompatibility of different vascular scaffold groups were studied by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), tensile test, burst pressure experiment, cytotoxicity experiment, endothelial cells planted in blood vessel scaffolds and hypodermic embedding of mice. The results showed that HLC-HA (0.05%) tubular scaffold exhibited interconnected well-distributed and porous structure and porosity of 94.38%; achieved the desirable mechanical property with stress of (1000.8±7.9) kPa and burst pressure of (1058.6±8.2) kPa, hypocytotoxicity, favourable cytocompatibility, hisocompatibility and disposition of degradation.