反义RNA技术在丝状真菌代谢工程中的应用

Applications of antisense-RNA technology in filamentous fungal metabolic engineering– a review

摘要:

丝状真菌作为一种重要的工业微生物, 采用各种表达调控技术对其代谢途径进行改造以便适应生产需求成为当前的研究热点之一。反义RNA技术是代谢工程中调控基因表达的一种重要手段, 且由于其操作简单避免了基因敲除技术的复杂性, 在丝状真菌体系中有着良好的应用前景。本综述中, 从反义RNA的作用机理、真菌体系的基因工程技术以及目前反义RNA技术的应用等方面, 对反义RNA技术在丝状真菌代谢工程中的应用进行了概述。

Abstract:

Filamentous fungi are important industrial microorganisms. The focus on its metabolic engineering is to optimize the metabolic pathway with gene expression regulation technology to meet with the industrial production needs. Antisense RNA technology due to its simplicity compared with the gene knock-out technology has great perspectives in filamentous fungal metabolic control. It is an efficient method for regulating gene expression and a key tool for metabolic engineering. In this article, we addressed the mechanism of antisense RNA technology and its applications in filamentous fungal metabolic engineering. Additionally, future perspectives were discussed.