门多萨假单胞菌Pseudomonas mendocina NK-01合成褐藻寡糖及其结构鉴定

Synthesis and characterization of alginate oligosaccharides produced by Pseudomonas mendocina NK-01

摘要:

研究发现门多萨假单胞菌NK-01在菌体内积累中长链聚羟基脂肪酸酯(PHAMCL)的同时也能够合成褐藻寡糖分泌到发酵液中, 其产量与培养基的碳氮比有关, 高碳氮比有利于褐藻寡糖的合成。本研究利用紫外-可见分光光度法、傅立叶红外光谱分析、1H和13C核磁共振对褐藻寡糖的结构进行了分析鉴定, 发现褐藻寡糖的结构是由b-D-甘露糖醛酸、a-L-古洛糖醛酸通过b-(1→4)/ a-(1→4)键连接而成的无支化线性多糖, 并且在单体的2位或3位羟基上部分乙酰化。凝胶渗透色谱(GPC)对分子量的测定结果为2054。

Abstract:

In this study, we found that when Pseudomonas mendocina NK-01 accumulated intracellular carbon reserve, medium chain length poly (3-hydroxyalkanoates), it also synthesized extracellular saccharides, alginate oligosaccharides. The high carbon nitrogen ratio of culture medium facilitated alginate oligosaccharides production. We analyzed the structure of alginate oligosaccharide by Ultraviolet-Visible Spectrophotometry, Fourier Transform Infrared Spectroscopy, 1H and 13C of Nuclear Magnetic Resonance, and found that it was compounded in line from β-D-mannuronic acids and α-L-gluronic acids via β-(1→4)/ α-(1→4) bonds, which acetylated partly on the 2- and/or 3-hydroxy. In addition, we determined the weight-average molecular weight of alginate oligosaccharides by gel permeation chromatography to be 2054.