两种藻胆蛋白对人肝癌SMMC-7721细胞的光动力体外杀伤效应

Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro

摘要:

用3-(4, 5-二甲基噻唑-2)-2, 5-二苯基四氮唑溴盐(MTT)法测定提取自条斑紫菜的R-藻红蛋白(R-PE)和C-藻蓝蛋白(C-PC)经激光介导对人肝癌SMMC-7721细胞的杀伤率, 并检测细胞凋亡率。结果显示在120 mg/L浓度下, R-PE在氩离子激光器100 J/cm2辐照下对应的细胞存活率为27%, C-PC在He-Ne激光器35 J/cm2辐照下对应的细胞存活率为47%; 单用2种激光器辐照对应的细胞存活率分别为65%和70%; 单用2种蛋白处理细胞在72 h后出现对细胞生长的显著抑制, 最大抑制率分别为31%(R-PE, 120 mg/L)和27%(C-PC, 250 mg/L)。120 mg/L的2种藻胆蛋白在对应激光辐照下, 于照后8 h 达到细胞凋亡率最大值, 分别为31.54%(R-PE)和32.54%(C-PC)。本实验证明条斑紫菜R-PE和C-PC具有可开发成为光敏剂的应用前景。

Abstract:

We studied the effect of photodynamic therapy with phycobiliproteins on human liver cancer cells in vitro. With 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay), we used two phycobiliproteins, R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) prepared from Porphyra yezoensis, to determine the killing rates and apoptosis rates of human liver cancer cells (SMMC-7721) mediated by laser. When the concentration of R-PE was 120 mg/L, the survival rate of human liver cancer cells was 27% after treated by Argon laser with 100 J/cm2 doses, while the survival rate in the control group (without adding R-PE) was 65%. When the C-PC concentration was 120 mg/L, the survival cell rate was 47% after treated by He-Ne laser with 35 J/cm2 dose, while the survival rate in the control group (without adding C-PC) was 70%. After handled only with these two kinds of phycobiliproteins for 72 h, the growth of cancer cells presented significant inhibition. The maximal inhibition rates reached up to 31% with R-PE (120 mg/L concentration) and 27% with C-PC (250 mg/L concentration) respectively. After irradiated by laser for 8 h, the maximal cell apoptosis rates were 31.54% with R-PE and 32.54% with C-PC, respectively. It indicated that R-PE and C-PC extracted from Porphyra yezoensis could develop to new photosensitizers for cancer photodynamic therapy.