一氧化氮和过氧化氢在内生真菌小克银汉霉属AL4诱导子促进茅苍术细胞挥发油积累中的作用

Role of nitric oxide and hydrogen peroxide in the essential oil increasing of suspension cells from Atractylodes lancea induced by endophytic fungal Cunninghamella sp. AL4 elicitor

摘要:

一株属于小克银汉霉属(Cunninghamella sp.)的内生真菌(编号为AL4)制成的粗诱导子可以诱发茅苍术悬浮细胞产生多种防卫反应, 包括一氧化氮(NO)、过氧化氢(H2O2)迸发和挥发油合成加强。NO专一性淬灭剂cPTIO和H2O2 淬灭剂过氧化氢酶(CAT) 则不仅可以分别抑制AL4 粗诱导子引起的茅苍术细胞的NO和H2O2 迸发, 还都能部分阻断AL4粗诱导子促进茅苍术细胞挥发油合成。添加NO供体硝普钠(SNP) 和H2O2 都可引起茅苍术细胞中挥发油积累增加, 但二者效果不同。因此暗示着NO 和H2O2 都是介导内生真菌AL4粗诱导子促进茅苍术悬浮细胞挥发油合成的信号分子。同时添加NO 的淬灭剂cPTIO 和H2O2 的淬灭剂CAT 并不能完全抑制AL4粗诱导子引起的茅苍术细胞挥发油积累增加, 这表明内生真菌AL4粗诱导子还可以通过其他方式促进茅苍术悬浮细胞挥发油合成。

Abstract:

Crude elicitor of one endophytic fungi (belong to Cunninghamella sp., named AL4) induced multiple responses in Atractylodes lancea suspension cells, including rapid generation of nitric oxide (NO) and hydrogen peroxide(H2O2), sequentially followed by enhancement of essential oil production. Adding NO-specific scavenger 2-4-carboxyphenyl-4,4,5, 5-tetramethylimidazol ine-1-oxyl-3-oxide (cPTIO) and H2O2 scavenger catalase (CAT) could block elicitor-induced NO and H2O2 generation respectively, but could all partly block elicitor-induced essential oil biosynthesis. Adding NO-donor sodium nitroprusside (SNP) and H2O2 could all promote essential oil accumulation in A. lancea cells, but the effect of both was different. These results strongly suggested that NO and H2O2 may all act as signaling molecule to mediate AL4 elicitor promoting essential oil accumulation in suspension cells of A. lancea. Furthermore, adding cPTIO and CAT contemporarily could not completely inhibit essential oil accumulation induced by AL4 elicitor. This result suggested that AL4 elicitor could also promote essential oil accumulation in suspension cells of A. lancea by other means.