Surfactin对人乳腺癌MCF-7细胞增殖、凋亡及细胞骨架的影响

Effects of surfactin on proliferation, apoptosis and cytoskeleton in human breast cancer MCF-7 cells

摘要:

以体外培养的人乳腺癌细胞株MCF-7为研究对象, 探讨surfactin对肿瘤细胞增殖、凋亡及细胞骨架的影响。MTT实验表明, surfactin能抑制MCF-7的增殖, 并且呈现出浓度和时间的依赖关系, 作用48 h时的IC50是27.3 mmol/L。AO/EB荧光染色法及流式细胞术检测发现, surfactin可诱导MCF-7发生典型的凋亡形态学改变和G2/M期阻滞。免疫荧光和免疫印迹结果表明, surfactin显著抑制了细胞内vimentin的表达, 诱导了a-tubulin的解聚和重排, 使细胞的骨架系统发生了剧烈的变化。可见, surfactin具有抑制MCF-7细胞增殖, 诱导细胞凋亡的作用, 其机制可能与细胞骨架蛋白的表达水平有关。

Abstract:

We studied the effect of surfactin on cell proliferation, apoptosis and the cytoskeleton in human breast cancer cell line MCF-7 in vitro. The result of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed that the surfactin inhibited proliferation of MCF-7 cells in a dose- and time-dependent manner, with IC50 at 48 h of 27.3 μmol/L. Surfactin-induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes by AO/EB staining. Flow cytometric analysis also demonstrated that surfactin caused time-dependent apoptosis of MCF-7 cells through cell arrest at G2/M phase. Immunofluorescence and Western blotting showed that surfactin significantly suppressed the expression of vimentin, induced the α-tubulin depolymerization and rearrangement and then the skeleton system of the cells changed dramatically. Based on our findings, surfactin can significantly inhibit the growth of MCF-7 cells and induce apoptosis.