低能离子束修饰蛹虫草菌株高产虫草素

High-yielding cordycepin in Cordyceps militaris modified by low-energy ion beam

摘要:

虫草素具有抗肿瘤、免疫调节、抗炎等多种功效。为了更好地开发蛹虫草资源, 选择合适剂量的低能离子束注入蛹虫草, 优化虫草素的提取工艺条件, 采用紫外分光光度法检测注入前后菌株中虫草素的含量。结果表明: 最佳注入剂量为2.60×1015 ions/cm2, 虫草素最佳的微波-超声波提取工艺为: 乙醇浓度70%, 提取功率200 W, 提取时间110 s, 料液比1:240。选育出虫草素含量较高的15株菌株, 最高含量达(11.924±0.063) mg/g, 比原始菌株增长了近30%。

Abstract:

Cordycepin has the effect of anti-tumor, immunomodulation, anti-inflammation and so on. In order to make use of Cordyceps militaris better, we implanted different doses of low-energy ion beam into C. militaris, chose best cordycepin extracting technology , and determined cordycepin content in strains before and after ion beam implantation by UV spectrophotometry. Results showed that the best dose of low-energy ion beam was 2.60×1015 ions/cm2, the best conditions of microwave assisted ultrasonic extraction technology were as follows: 70% ethanol as the solvent, microwave power as 200 W, extraction time as 110 s, Material - liquid ratio as 1: 240. We chose 15 strains with high-yielding cordycepin, of which cordycepin content was up to (11.924±0.063)?mg/g, which was a nearly 30% increase compared with the original strain.