圆红冬孢酵母两阶段培养法生产微生物油脂

Microbial lipid production by Rhodosporidium toruloides in a two-stage culture mode

摘要:

为缩短发酵时间，减少原料消耗，采用细胞增殖和油脂积累分离的两阶段模式，培养圆红冬孢酵母Rhodosporidium toruloides AS 2.1389生产微生物油脂。结果表明，细胞增殖阶段获得的R. toruloides AS 2.1389细胞，重悬接种在葡萄糖溶液中，可快速积累油脂，菌体油脂含量超过自身干重的55％。增殖阶段细胞的菌龄越高，产油能力越强。油脂积累阶段使用高浓度葡萄糖溶液或未灭菌的葡萄糖溶液，油脂合成可以有效进行。油脂中脂肪酸成分以含16和18个碳原子的长链脂肪酸为主，可作为制备生物柴油的新型原料。

Abstract:

To shorten the cultivation time and reduce the consumption of raw materials for microbial lipid production, oleaginous yeast Rhodosporidium toruloides AS 2.1389 was cultivated using a two-stage culture mode, in which the cell propagation and lipid accumulation were separated. The yeast cells recovered from the propagation culture were re-suspended in glucose solution for lipid accumulation, through which lipid content over 55% of the dry cell weight was achieved, the longer the propagation stage was, the higher the lipid content. Analysis of the lipid indicated that the long-chain fatty acids with 16 and 18 carbon atoms were major components, suggesting that the lipid can be an alternative feedstock for biodiesel production.