基于矿石纳米材料的DNA转化的机理初探及方法改进

Mechanism of DNA transformation based on mineral nanofibers and method improvement

摘要:

一种价格便宜、资源丰富和对人体无害的矿石纳米材料——海泡石，用于微生物DNA转化。该法简便、快速、对人体健康无害，但对这种转化方法机制的理解还有很多问题。通过小片段RNA竞争试验，发现了与先前报道不同的转化机制。同时，对该法进行了优化，结果可以实现对冷藏1个月的大肠杆菌Escherichia coli DH5α单菌落直接转化，无需感受态制备和处理后的温育过程，可得到比钙转高的转化率。由于可优化的参数很多，所以这种转化方法可以提升的空间很大。同时，该方法可用于探索其他用钙转和电转未成功的微生物。

Abstract:

Sepiolite?an inexpensive, resourceful, fibrous yet inoffensive mineral?made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competition test, we proposed the different transforming mechanisms from the previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4°C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.