常压室温等离子体快速诱变产油酵母的条件及其突变株的特性

Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants

摘要:

采用新型常压室温等离子体射流诱变产油酵母，结合快速突变产油酵母操作条件及基于96孔板的高通量筛选手段，获得了一系列增殖速度和产油量发生变化的突变株。在等离子体对菌株致死率为99％的条件下获得的以突变株增殖速度为指标的正突变率达到27.2％。用含酵母粉 (10 g/L)、蛋白胨 (10 g/L) 及葡萄糖 (20 g/L) 的酵母膏胨葡萄糖培养基进行发酵实验表明，筛选得到的高产突变体产油量从对照株的1.87％ (W/W) 增加到4.07％ (W/W)。

Abstract:

To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma (ARTP) jet was used with a 96-well plate for high throughput screening. Mutants with changes in growth rates and lipid contents were obtained. At a lethality rate of 99%, the positive mutation rate of the yeast cells was 27.2% evaluated by the growth rates of the mutants and the comparison with the wild strain. The fermentation in a medium composed of yeast extract (10 g/L), peptone (10 g/L) and D-glucose (20 g/L) resulted in the lipid yield of the mutant (C4) with 4.07% (W/W) compared with that of the wild strain (1.87%).