利用转座标签mTn-lacZ/leu2插入突变发酵性丝孢酵母2.1368-Leu?筛选高效产油突变株

Screening of high lipid production Trichosporon fermentans mutants by transposon tagging mTn-lacZ/leu2 insertion

摘要:

为提高微生物油脂产率，降低其生产成本，以转座标签mTn-lacZ/leu2插入突变发酵性丝孢酵母2.1368-Leu?筛选高效产油突变株。利用LacZ显色反应、脂肪酸合成酶抑制剂Cerulenin和磷酸香草醛反应，最终在玉米秸秆糖化液中筛选出一株高效产油突变株2.1368-Leu?-7。结果表明其油脂含量为38.30％，比对照的29.33％高了8.97％，而其产油率为8.35％，比对照的6.92％提高了20.63％；在玉米秸秆糖化液中的糖利用率为77％，每100 g玉米秸秆可转化油脂8.32 g。可为未来生物柴油产业提供了廉价原料。

Abstract:

To improve microbial lipid production, we inserted mTn-lacZ/leu2 into Trichosporon fermentans 2.1368-Leu? to obtain high lipid production mutants. By observing the LacZ chromogenic change, the positive reaction between Cerulenin (inhibitor of fatty acid synthase) and phosphate vanillin, a higher lipid-producing mutant 2.1368-Leu?-7 grown on corn-stalk hydrolysate was obtained. The lipid content of this mutant reached 38.30% (8.97% higher than that of the control) and the lipid production rate was 8.35% (20.63% higher than that of the control). The rate of sugar utilization was 77%, meaning that 100 g corn-stalk could be converted to 8.32 g lipid. The study provided an effective method for microbial lipid production by using cheap raw materials for biodiesel.