DNA稳定同位素探针技术在宏基因组学中的应用及前景

Applications and perspectives of DNA stable-isotope probing in metagenomics: a review

摘要:

DNA稳定同位素探针 (DNA-SIP) 是一种新兴的技术，通过将同位素稳定结合到特定的底物来确定环境中微生物的作用。DNA-SIP与宏基因组学结合可以让某些微生物的特性与其特殊新陈代谢联系在一起，不仅可以从宏基因组库里检测到低含量的微生物，而且加速了对新的酶类和其他生物活性物质的发现。以下总结了SIP-宏基因组学技术的原理、应用及研究进展，并讨论了其在环境微生物学和生物技术的应用前景。

Abstract:

DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds. We summarize recent progress in SIP-metagenomic techniques and applications and discuss prospects for this combined approach in environmental microbiology and biotechnology.