海藻工具酶——褐藻胶裂解酶研究进展

Advances in algae toolenzymes: alginate lyases

摘要:

从海洋生物中筛选提取有价值的酶类，开发海洋多糖降解产物，已成为海洋生物资源开发的一个重要方面。因此，近年来对于海藻工具酶之一的褐藻胶裂解酶及其降解产物——褐藻寡糖的研究日益受到人们的普遍关注。从褐藻胶裂解酶的来源、分类、底物专一性、作用方式及结构与机理研究、酶活力测定和酶学性质等方面，结合本课题组的研究工作综述近十年来有关褐藻胶裂解酶的研究进展。

Abstract:

Marine can be considered as a rather unexplored source of biological material. Production of algal oligosaccharides by using valuable enzymes from marine origin has become an important way to utilize marine resources. As one of algal tool enzymes, the use of alginate lyases has been focused mainly on development and application of alginate oligosaccharides with bioactive function in recent years. In this paper, we reviewed the research of alginate lyases over the past decade in several aspects, including their origin, diversity, substrate specification, mode of action, structure and catalysis mechanism, assay of enzyme activity, enzyme characterization, as well as our own experience on this subject. At the end of the review, the application prospects of alginate lyases are presented.