脂肪干细胞的共培养及其分化应用概述

Progress in co-culture and differentiation in adipose-derived stem cells: a review

摘要:

干细胞在体外特定培养条件下可以被诱导分化成具有不同体细胞表型的细胞。除了通过不同培养条件进行体外诱导分化的方法外，用成熟体细胞与干细胞共培养同样可以诱导干细胞定向分化。以下首先简述了脂肪干细胞 (Adipose-derived stem cells，ADSCs) 的来源及其标志，然后重点就ADSCs的不同培养方法、诱导分化及最新的临床应用进行阐述，包括药物及化学诱导培养、体细胞与ADSCs二维、三维共培养等，最后提出ADSCs的问题所在并对此技术进行展望。

Abstract:

Stem cells can be differentiated into many kinds of somatic cells under defined culture conditions. In addition, the homing possess can be partially imitated by co-culture of stem cells with mature somatic cells. Regarding the importance of clinical application of adipose-derived stem cells (ADSCs), our review first introduced the sources and signs of ADSCs, and then the current knowledge of ADSCs co-culture technology, including drug and chemical induced culture, two-dimensional (2D) and three-dimensional (3D) co-culture, mechanisms of ADSCs differentiation, and application development in recent years in details. Finally, we also addressed prospects of ADSCs.