果糖和麦芽糖作为有氧碳源对大肠杆菌NZN111两阶段发酵中厌氧发酵的影响

Effects of fructose and maltose as aerobic carbon sources on subsequently anaerobic fermentation by Escherichia coli NZN111

摘要:

为了了解磷酸转移酶转运系统 (PTS) 依赖和非PTS依赖代谢的糖类对大肠杆菌生产琥珀酸的影响，进行了两阶段培养，有氧阶段采用PTS依赖型的果糖或非PTS依赖型的麦芽糖作为丙酮酸甲酸裂解酶 (PFL) 和乳酸脱氢酶 (LDH) 双突变株NZN111的碳源，研究其对NZN111厌氧阶段代谢葡萄糖的影响。5 L罐发酵结果表明，以果糖和麦芽糖为碳源有氧培养的细胞恢复了在厌氧条件下快速代谢葡萄糖的能力，琥珀酸和丙酮酸成为主要代谢产物，最终琥珀酸得率分别为0.84和0.75 mol/mol，丙酮酸得率分别达到了0.65和0.83 mol/mol，琥珀酸和丙酮酸终浓度比分别为1.73∶1和1.21∶1。果糖和麦芽糖培养的NZN111与葡萄糖培养的菌体代谢的明显差异推测是cyclic AMP (cAMP) 依赖型和非cAMP依赖型的分解代谢物阻遏调控这两种机制共同作用的结果。

Abstract:

To understand the effects of sugar whose uptake is dependent or independent on the phosphotransferase system (PTS), two-stage culture of Escherichia coli strain NZN111 that was constructed by disruption of ldhA and pflB encoding the fermentative lactate dehydrogenase (LDH) and pyruvate: formate lyase (PFL) of E. coli W1485, was carried out for organic acids production. When NZN111 was aerobically cultured on fructose (PTS dependent) or maltose (PTS independent), it fermented glucose with succinic acid and pyruvic acid as the major products in subsequent anaerobic culture. The experiments were also performed in a 5-L fermentor. The yields of succinic acid by the fructose-and maltose-grown NZN111 were 0.84 and 0.75 mol/mol, whereas the yields of pyruvic acid were 0.65 and 0.83 mol/mol, respectively. The final ratio of succinic acid to pyruvic acid in the anaerobic stage reached 1.73:1 and 1.21:1, respectively. The different behaviors in anaerobic fermentation by the fructose-, maltose- and glucose-grown NZN111 were likely caused by the regulation of catabolite repression in the aerobic culture stage.