细胞表达流感病毒抗体CR6261 VH与scFv对流感病毒感染的影响

Effect of antibody CR6261 VH and scFv expression on influenza virus infection

摘要:

由于新发现的流感病毒抗体CR6261可以中和多种亚型的流感病毒，而且是其VH区结合于流感病毒蛋白HA的保守区，因此该抗体有望成为一种广谱的流感治疗性抗体而备受关注。通过构建真核表达载体pCR6261VH、pCR6261VH-GFP、pCR6261scFv，成功地筛选到在细胞膜上稳定表达CR6261VH、CR6261VH-GFP与CR6261scFv的单克隆细胞系。用流感病毒去感染稳定细胞系，间隔12 h取上清检测病毒血凝效价。结果表明：稳定表达CR6261scFv和CR6261VH-GFP细胞系能够降低病

Abstract:

The emerging CR6261 antibody could neutralize several subtype influenza virus with high affinity, whose VH domain binds to the HA protein conserved domain. Therefore, it has drawn much attention as a potential broad-spectrum therapeutic antibody against influenza virus. In this study, we constructed the eukaryotic expression vectors pCR6261VH, pCR6261VH-GFP and pCR6261scFv and screened the monoclonal cell lines that could stably express CR6261VH, CR6261VH-GFP and CR6261scFv on the cell membrane. After influenza virus infecting the stable cell lines, the titers of viruses were tested by hemagglutination inhibition test. The result shows that the titers of viruses in CR6261scFv and CR6261VH-GFP stable expression cell lines decreased and there was no obvious discrimination between the CR6261VH expression cell line and the negative control, suggesting that CR6261VH and CR6261scFv expressing on the cell membrane could partly inhibit the virus infection. Though the effective inhibition strategy is undergoing, our research will provide new clues for the breeding of anti influenza transgenic animals.