利用嗜盐菌Salinivibrio YS生产2,3-丁二醇和琥珀酸

Production of 2, 3-butanediol and succinic acid by Salinivibrio YS

摘要:

以从新疆艾丁湖采集的土样中分离出的中度嗜盐菌Salinivibrio YS为研究对象，利用该菌在厌氧条件下生产2,3-丁二醇和琥珀酸，在单因素摇瓶实验基础上，确定影响产物积累的各因素及其相应条件，再利用正交试验确定这些参数的最佳水平，即温度33 ℃，起始pH为8.0，发酵过程pH为7.0，乙酸添加量为3 g/L，NaCl浓度为10 g/L。利用优化条件进行3 L体系的发酵放大实验，经过108 h的无氧发酵，2,3-丁二醇的产量可达35.05 g/L，而琥珀酸的含量则高达22.46 g/L，且其糖的总转化率高达约50％。首次利用嗜盐菌在厌氧条件下生产2,3-丁二醇和琥珀酸，拓展了嗜盐菌的应用，同时也为生产2,3-丁二醇和琥珀酸提供了新的思路。

Abstract:

The production of 2, 3-butanediol and succinic acid by a moderate halophile under anaerobic condition was investigated. This halophile, termed Salinivibrio YS, was isolated from the solid samples collected from Aydingkol Lake. Based on the single factor experiment, the parameters and their values for the production were obtained. Then, the optimum values of these parameters by the orthogonal experiments were obtained: temperature, 33 °C; initial pH of fermentation, 8.0; the pH during fermentation, 7.0; the concentration of acetic acid was 3 g/L and NaCl was 10 g/L. Finally, a 3-L fermentation based on these conditions was carried out. After 108 h of fermentation under anaerobic condition, 35.05 g/L of 2, 3-butanediol and 22.46 g/L of succinic acid were obtained. About 50% of total glucose conversion was achieved. The study on 2, 3-butanediol and succinic acid by a halophile under anaerobic condition will expand the applications of halophiles and open a new area of production of 2, 3-butanediol and succinic acid.