α半水硫酸钙复合功能化多壁碳纳米管骨修复材料生物相容性

Biocompatibility of α-calcium sulfate hemihydrate (CSH)/multi-walled carbon nanotube (MWCNT) composites for bone reconstruction application

摘要:

旨在评价α半水硫酸钙复合功能化多壁碳纳米管骨修复材料的生物相容性，为后期临床实验奠定基础。分别采用兔L929成纤维细胞与材料浸提液以及材料片剂与兔骨髓基质干细胞复合培养，在不同时段运用倒置显微镜观察，MTT检测，扫描电子显微镜分析等手段，观察材料对细胞的相容性；进行急性、亚急性毒性实验，肌肉植入实验，骨植入实验等，观察材料对组织的相容性。结果表明，L929成纤维细胞在材料浸提液中生长良好，MTT检测与对照组无显著差异 (P＞0.05)；扫描电子显微镜结果显示，细胞能在材料上黏附并增殖；体内实验表明材料对机体无毒，无致敏性，组织相容性佳。α半水硫酸钙/碳纳米管复合材料表现出的良好生物相容性，有望在骨组织工程中得到广泛运用。

Abstract:

We examined the biocompatibility and the safety of α-calcium sulfate hemihydrate (CSH)/multi-walled carbon nanotube (MWCNT) composites for bone reconstruction application. The biocompatibility of the CSH/MWCNT composites was evaluated by the measures which taking L929 fibroblast cells cultured in the extracted liquid of the composite soaking solution and putting bone marrow stromal cells planted on the composite pellets in vitro, respectively. The cell proliferation was evaluated by MTT test and further observed using an inverted optical microscope and a scanning electric microscope. The toxicity of the composites was evaluated by acute and subacute systemic toxicity test. Long-term muscle and bone implantation in vivo tests were also conducted. L929 fibroblast cells grew well in the extracted liquid, as well as bone marrow stromal cells that could adhere on the surface of sample pellets and proliferated rapidly. MTT test showed that there were no significant differences between the experimental and control groups (P>0.05). In vivo test manifested that the composites were no toxicity, no irritation to skin and good for bone defect reconstruction. It was proved that α-calcium sulfate hemihydrate (CSH)/multi-walled carbon nanotube (MWCNT) composites exhibited excellent biocompatibility for the potential application in bone tissue engineering.