利用温度调节实现新型重组菌高效转化甘油为D-乳酸

Temperature-switched high-efficiency D-lactate production from glycerol

摘要:

油脂水解来源的甘油将是未来发酵工业主要原料之一。文中探索D-乳酸高产大肠杆菌Escherichia coli CICIM B0013-070菌株不同培养温度下好氧与厌氧代谢甘油的特征后，建立并优化了一种新型D-乳酸变温发酵工艺，甘油到乳酸的得率由64％提高到82.6％。另外，在B0013-070中引入了温度诱导型乳酸脱氢酶的转录系统，甘油到乳酸的得率进一步提高到88.9％。

Abstract:

Glycerol from oil hydrolysis industry is being considered as one of the abundent raw materials for fermentation industry. In present study, the aerobic and anaerobic metabolism and growth properties on glycerol by Esherichia coli CICIM B0013-070, a D-lactate over-producing strain constructed previously, at different temperatures were investigated, followed by a novel fermentation process, named temperature-switched process, was established for D-lactate production from glycerol. Under the optimal condition, lactate yield was increased from 64.0% to 82.6%. Subsequently, the yield of D-lactate from glycerol was reached up to 88.9% while a thermo-inducible promoter was used to regulate D-lactate dehydrogenase transcription.