重离子诱变创制高产油微拟球藻新品种

Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis

摘要:

以具有产业化前景的微拟球藻Nannochloropsis oceanica OZ-1为实验材料，利用碳重离子进行诱变育种，采用Imaging-PAM和酶标仪进行大规模筛选，最终获得两株高生长速率微拟球藻突变藻株 (HP-1和HP-2)，进一步分析显示两株突变藻株 (HP-1和HP-2) 生物量积累较野生型藻株大幅提高，在18 d培养末期生物量分别提高了18％和26％，两株突变藻株油脂产率分别为295 mg/(L·d)和275 mg/(L·d)，而野生型藻株为247 mg/(L·d)。所获两株突变藻株生长速度快、油脂产率高，较野生型藻株优势明显。

Abstract:

Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 19% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L·d) and 275 mg/(L·d), respectively, whereas that of the wild type was 247 mg/(L·d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.