免疫磁珠富集技术联合选择性培养基快速检测单增李斯特菌

Rapid detection of Listeria monocytogenes by immunomagnetic separation combined with selective medium

摘要:

单增李斯特菌是一种危害极大的食源性致病菌，建立快速及特异的检测方法对于食品安全监控尤为重要。文中联合免疫磁珠与选择性培养基对不同浓度 (101~105 CFU/mL) 单增李斯特菌进行检测，并对3种李斯特菌、金黄色葡萄球菌及副溶血弧菌进行交叉试验；同时模拟食物污染，探索免疫磁珠-平板法检测样品的检测限以及该方法的最快检测时间。结果显示特异性免疫磁珠联合选择性平板法可检出浓度为103 CFU/mL及以上的单增李斯特菌；牛奶样品仅需6 h增菌能被检出，检测限为0.7 CFU/mL。联合使用免疫磁珠富集技术与选择性培养基，能在30 h内完成对牛奶样品的检测，较国标法减少38 h以上，且具有同等的灵敏度。

Abstract:

Listeria monocytogenes is a pathogenic bacterium, therefore, it is essential for food safety monitoring to establish a rapid and specific detecting method. In this study, immunomagnetic beads and selective medium were combined to detect Listeria monocytogenes at different concentrations (101?105 CFU/mL). Other three types of Listeria spp., Staphylococcus aureus and Vibrio parahaemolyticus were also detected to conduct the cross-reaction analysis. Meanwhile, contaminated milk samples were prepared to explore the limit of detection of immunomagnetic beads combining with selective medium. Results showed that Listeria monocytogenes with the concentration of 103 CFU/mL and above was successfully detected. Milk samples were detected within 6 hours, with a detection limit of 0.7 CFU/mL. The method developed is capable of detecting milk samples within 30 h, which is 38 h faster compared with national standard method with the same sensitivity.