高效转化黄姜皂苷为薯蓣皂苷元菌株的筛选及转化条件优化

Screening and condition optimization of a strain for efficiently biotransformation of saponins in Dioscorea zingiberensis into diosgenin

摘要:

薯蓣皂苷元是甾体激素类药物重要的生产原料，在制药工业中有广泛应用。传统的生产方法是黄姜酸解法，污染严重。为寻找更清洁高效的生产方式，从本实验室保藏的菌株中筛选出一株赤霉菌Gibberella intermedia WX12 (层出镰孢菌Fusarium proliferatum的有性阶段)，能将黄姜中的皂苷转化为薯蓣皂苷元。采用统计学实验设计方法对其转化培养基进行研究，优化的转化培养基配方为 (g/L)：葡萄糖20.6；酵母膏5.0；氯化钠1；磷酸二氢钾3；硫酸锌1.5；黄姜酶解物3。采用以上优化参数，薯蓣皂苷元的得率提高到 (31±0.3) mg/g干黄姜，较优化前提高了3倍。这是目前关于赤霉菌转化黄姜中皂苷的首次报道。

Abstract:

Diosgenin is an important raw material in steroid hormone and widely used in pharmaceutical industry. The traditional method for diosgenin production is acidolysis, which causes serious pollution. In order to obtain a cleaner and more efficient approach of diosgenin production, a strain of Gibberella intermedia WX12 (the sexual stage of Fusarium proliferatum) was screened from the strains deposited in our laboratory. This strain converted saponins in Dioscorea zingiberensis C.H. Wright (DZW) into diosgenin. The conversion medium was optimized by statistical experimental design. The optimized conversion medium was as follows (g/L): glucose 20.6, yeast extract 5.0, NaCl 1, K2PO4 3 , ZnSO4·7H2O 1.5 and saponins 3. Under the optimal conditions, the yield of diosgenin achieved to (31±0.3) mg/g DZW, which was 3 times higher than that of the original medium.