β-1,3-葡甘露聚糖酶辅助提取圆红冬孢酵母油脂

β-1,3-glucomannanase assisted lipid extraction from Rhodosporidium toruloides

摘要:

为考察β-1,3-葡甘露聚糖酶辅助提取圆红冬孢酵母Rhodosporidium toruloides油脂工艺的应用潜力，以R. toruloides Y4发酵醪液为原料，乙酸乙酯为溶剂，在10 L规模下研究了酶解和萃取条件对油脂提取率的影响，并进行了初步物料平衡分析。结果表明：酶处理0.5 h，油脂提取率可达71.1%，酶解效率与10 mL小体系所取得的结果相当。通过多次萃取，有效缓解了乳化现象对油脂提取的影响。经过3次萃取，油脂提取率、溶剂回收率和总物料回收率分别达92.9%、87.0%和94.2%。酶辅助提取油脂工艺可直接利用油脂发酵醪液，提取率高，对设备要求低，具有很好的工业化应用潜力。

Abstract:

To evaluate the effectiveness of enzymatic assisted extraction (EAE) of lipid from the oleaginous yeast Rhodosporidium toruloides in the presence of β-1,3-glucomannanase at a larger scale, we investigated the effects of enzymatic treatment and extraction conditions on lipid extraction yields at 10-L scale by using the broth of R. toruloides Y4 as the feed and ethyl acetate as the solvent. When it was treated for 0.5 h, the lipid extraction yield reached 71.1%, indicating that the enzymatic treatment process reached similar efficiency to that obtained at 10-mL scale. The inhibitory effect of emulsification was greatly reduced by repeated extraction. After extracted for three times, yields of lipid extraction, solvent recovery and total material recovery reached 92.9%, 87.0% and 94.2% respectively. As it can use the lipid production slurry with good extraction efficiency, EAE technology is promising for industrial production of microbial lipids.