利用农业废弃物固态发酵裂褶菌F17产锰过氧化物酶的基质优化

Optimization of manganese peroxidase production from Schizophyllum sp. F17 in solid-state fermentation of agro-industrial residues

摘要:

锰过氧化物酶 (MnP) 在环保领域有着广阔的应用前景。目前，利用廉价基质生产MnP，尤其是利用工农业废弃物生产MnP的研究受到了国内外学者的广泛关注。本实验利用响应面方法从几种不同的农业废弃物中筛选裂褶菌F17 (Schizophyllum sp. F17) 产MnP的固态发酵基质。结果表明，以0.52∶0.15∶0.33的比例组成的松木屑、稻草和黄豆粉的混合基质为发酵产MnP的最佳基质，发酵第6天MnP的活力最高，达到11.18 U/g。因此，利用农业废弃物固态发酵产锰过氧化物酶在减低酶的成本和环境污染物治理方面具有重要的意义。

Abstract:

Manganese peroxidase (MnP), a crucial enzyme in lignin degradation, has wide potential applications in environmental protection. However, large-scale industrial application of this enzyme is limited due to several factors primarily related to cost and availability. Special attention has been paid to the production of MnP from inexpensive sources, such as lignocellulosic residues, using solid-state fermentation (SSF) systems. In the present study, a suitable SSF medium for the production of MnP by Schizophyllum sp. F17 from agro-industrial residues has been optimized. The mixed solid medium, comprising pine sawdust, rice straw, and soybean powder at a ratio of 0.52:0.15:0.33, conferred a maximum enzyme activity of 11.18 U/g on the sixth day of SSF. The results show that the use of wastes such as pine sawdust and rice straw makes the enzyme production more economical as well as helps solve environmental problems.