激光显微切割/质谱联用技术在肾脏疾病诊断中的应用进展

Laser microdissection and mass spectrometry based proteomics in the diagnosis of kidney diseases

摘要:

激光显微切割 (Laser microdissection，LMD) 质谱 (Mass spectrometry，MS) 联用技术 (LMD/MS) 已成功应用于肾活检组织甲醛固定石蜡包埋切片的蛋白质组学研究，提高了某些肾脏病的诊断水平，显示出较好的临床应用前景。文中就LMD/MS蛋白质组学技术的原理、方法及该技术在肾淀粉样变性、膜增殖性肾小球肾炎等肾脏疾病的发病机制及诊断分型的应用进展进行综述。

Abstract:

In recent years, laser microdissection followed by mass spectrometry (LMD/MS) has been successfully applied to the proteomic studies of formalin-fixed paraffin-embedded (FFPE) renal tissues. This new technique improves the diagnosis of kidney diseases and has a better potential for future clinical application. The review focuses on the use of this methodology for exploring the mechanisms, diagnosis and classification of kidney diseases including renal amyloidosis and membrane proliferative glomerulonephritis.