去分化脂肪细胞的研究进展

Progress in dedifferentiated fat cells

摘要:

去分化脂肪 (Dedifferentiated fat，DFAT) 细胞是由人体内含量最丰富的成熟脂肪细胞经体外天花板法培养去分化而来。研究发现：DFAT细胞具有均一性高、对供者年龄要求较低等脂肪来源干细胞 (Adipose-derived stem cells, ASCs) 和骨髓间充质干细胞 (Bone marrow mesenchymal stem cells，BMSCs) 所不具有的优势。此外，它还具有体内外成脂、成软骨、成骨、成肌、成神经等多向分化能力以及免疫调节能力。作为具有潜力的组织工程及同种异体干细胞移植的优秀种子细胞，DFAT细胞在治疗骨缺损、神经性疾病、局部缺血性心脏病及肾脏疾病等方面均具有较好的应用前景，对其开展深入的研究具有重要的理论和实践意义。文中从免疫学性质、多向分化能力及临床应用潜力等方面对DFAT细胞的研究进展作一综述。

Abstract:

When mature adipocytes are subjected to an in vitro dedifferentiation strategy referred to as ceiling culture, these mature adipocytes can revert to dedifferentiated fat (DFAT) cells. DFAT cells have many advantages compared with adipose-derived stem cells (ASCs) and bone marrow mesenchymal stem cells (BMSCs). For example, DFAT cells are homogeneous and could be obtained from donors regardless of their age. Furthermore, DFAT cells also have the same multi-lineage potentials and low immunogenicity as ASCs. As an excellent source of seed cells for tissue engineering and stem cell transplantation, DFAT cells have better prospects in the treatment of many clinical diseases, such as bone defects, neurological diseases, ischemic heart disease and kidney disease. It is necessary to make more intensive studies of DFAT cells. This article summarizes progresses in the immunological characteristics, differentiation ability and potential clinical applications of DFAT cells.