大肠杆菌全局调控转录因子环腺苷酸受体蛋白质在代谢工程中的应用

Regulation of global transcriptional factor cyclic amp receptor protein and its metabolic engineering application in Escherichia coli

摘要:

环腺苷酸受体蛋白质 (cyclic amp receptor protein, CRP) 是原核生物共有的一类全局转录因子，调控原核生物大肠杆菌Escherichia coli中近一半基因的转录。通过易错PCR或DNA shuffling的方法可以获得CRP突变体文库，然后经过筛选可以得到目的细胞表型——“抗逆性”增强。本研究综述了突变CRP在细胞表型：耐氧化压力、耐渗透胁迫、耐有机溶剂 (甲苯)、发酵中耐醋酸盐类和生物醇生产中耐生物醇中的应用。推论出CRP同样可以应用于相似微生物的表型培育，并展望了CRP的巨大应用潜力。

Abstract:

Cyclic amp receptor protein (CRP) is a global transcriptional factor in many prokaryotes, capable of governing nearly half of the total genes in Escherichia coli. Through the method of error-prone PCR or DNA shuffling, we can first obtain CRP mutant library and then get the expected cell phenotype with enhanced resistance. In this article, we reviewed the following desired phenotype: enhanced tolerance towards oxidative stress, improved osmotolerance, enhanced organic solvent (toluene) tolerance, improved acetate tolerance of E. coli fermentation and improved ethanol tolerance during bio-ethanol production. We then concluded that CRP can also be applied in other host cells to get desired phenotypes. Last, we predicted potential applications of mutant CRP transcriptional factor.