大孔树脂吸附与反相色谱纯化恩拉霉素

Purification of enramycin by macroporous resin adsorption and reversed phase chromatography purification

摘要:

恩拉霉素作为多肽类抗生素，是一种新型、安全的饲料添加剂。本文建立了一条基于大孔树脂初纯和反相色谱精制的分离纯化工艺。该工艺路线首先使用AB-8大孔树脂在0.012 mol/L盐酸溶液-甲醇 (50：50，V/V) 缓冲液条件下洗脱实现恩拉霉素初步纯化，再使用制备型C18反相色谱柱在0.05 mol/L 磷酸二氢钠-乙腈 (70：30，V/V) (pH 4.5) 缓冲液洗脱下实现恩拉霉素a和b的有效分离，a、b两个组分纯度分别达到98.5%和98.0%，a和b两种有效成分的总收率为29.2%。本研究为恩拉霉素a和b两种纯品的制备以及高纯度恩拉霉素产品的生产提供了参考。

关键词:恩拉霉素，大孔吸附树脂，反相色谱，纯化

Abstract:

Enramycin is a polypeptide antibiotic and new, safe animal feed additive. A new purification process was developed, based on pre-purification by macroporous resin and refining by reversed phase chromatography. AB-8 macroporous resin was used for the pre-purification process of enramycin, with an elution buffer of 0.012 mol/L aqueous HCl solution-methanol (50: 50, V/V). Then, enramycin a and enramycin b were separated effectively by C18 reversed phase chromatography, with a elution buffer of 0.05 mol/L aqueous KH2PO4 solution-acetonitrile (70: 30, V/V, pH 4.5). The purities of enramycin a and enramycin b were up to 98.5% and 98.0%, respectively. The yield reached 29.2%. This study would provide a useful reference for the preparation of enramycin a and enramycin b with ahigh purity.

Key words:enramycin, macroporous adsorption resin, reverse-phase chromatography, purification