转Bt基因抗虫作物培育现状及Bt蛋白的改造和聚合策略的利用

Bt transgenic crops for insect-resistance and modification of Bt protein and utilization of stacking strategy

摘要:

Bt基因是目前世界上应用最为广泛的抗虫基因，已经被转入多种作物中。其中，棉花、玉米、马铃薯等转Bt基因抗虫作物已经商品化生产，创造了可观的经济效益。结合作者的资料收集和研究结果，综述了Bt基因以及转Bt基因抗虫作物的培育现状，同时对提高Bt蛋白杀虫活力的方法和Bt基因聚合策略的利用进行了深入的探讨。

Abstract:

Insecticidal protein genes from Bacillus thuringiensis are currently the most widely used insect-resistant genes. They have been transferred to many crops for breeding and production. Among them, cotton, maize, potato and other insect-resistant crops are commercialized, creating considerable economic benefit. In this review, we summarized advances in identifying functional genes and transgenic crops for insect resistance, compared different strategies for enhancing vigor of insecticidal protein and utilizing gene stacking as well as listing valuable groups of stacked genes. In addition, the methods for multiple gene transformation was discussed.