聚N-异丙基丙烯酰胺细胞培养平台的研究进展

Advances in poly (N-isopropylacrylamide) based platforms for cell culture

摘要:

聚N-异丙基丙烯酰胺 (poly(N-isopropylacrylamide), PNIPAAm)，温敏性聚合物，可利用其温敏特性替代酶类物质或细胞刮刀用于贴壁细胞的收获，从而有效避免酶解和机械损伤，可为生物医药领域提供品质优良的种子细胞。重点阐述促进细胞有效粘附和快速脱附的温敏性PNIPAAm二维平面的研发情况，包括选取特殊基材、引入亲水基团、调节反应物比率、控制聚合物厚度/密度、提供适宜外力等方式，从而有效改善细胞对温敏性平面的适应性并降低染菌风险以及减少低温处理对细胞的影响。同时介绍PNIPAAm微载体、支架和凝胶等温敏性三维培养介质的研究进展，此方式不仅增加细胞生长面积，更可以模拟体内微环境，从而保持细胞原始生理特征，同时实现大规模扩增和非酶解收获细胞以及组织器官修复和重构的目标。最后简单说明PNIPAAm培养平台的应用，PNIPAAm的研发为再生医学的发展提供了崭新思路。

Abstract:

Poly (N-isopropylacrylamide) (PNIPAAm), a temperature-responsive polymer, can be potentially applied to replace enzymes or cell scrapers to recover attached cells. Taking full advantage of this unique function of PNIPAAm, cells can be protected from enzymatic hydrolysis and mechanical treatment, thereby to provide ideal seed cells with high quality for biomedical fields. In this review we describe the method to facilitate cell effective adhesion and rapid detachment on thermoresponsive two dimensional surfaces, including selecting special substrate, introducing hydrophilic group, adjusting reactant ratio, controlling polymer thickness/density, providing appropriate external force, so as to effectively improve adherent cell adaptability to thermoresponsive surfaces, depress the risk of bacterial contamination and reduce the effect of low-temperature treatment on the cells. The three dimensional cell culture systems involved in temperature-sensitive microcarriers, scaffolds and gels were briefly discussed. The application based on the platforms for cell culture was also presented.