黑曲霉发酵过程中菌体形态的分析方法建立及应用

Development and application of morphological analysis method in Aspergillus niger fermentation

摘要:

丝状真菌 (Filamentous fungi) 的发酵生产通常具有较高的工业应用价值，但其菌体形态是一个区别于其他非丝状菌的一个重要发酵指标。针对目前形态分析的瓶颈，本研究使用琼脂糖凝胶对黑曲霉菌形进行固定，利用平板实现菌球样本的大量制备，并结合图形处理软件自建自动化处理程序，实现了大量准确可靠的菌体形态参数的获得，大大增加了形态数据处理通量及准确度。应用该方法于黑曲霉发酵生产糖化酶过程中不同供氧水平及剪切水平下菌体形态的研究，通过大量形态数据定量阐明了黑曲霉在不同剪切水平下的分区域形态分布特性，为进一步工业过程的形态优化提供了重要的研究方法。

Abstract:

Filamentous fungi are widely used in industrial fermentation. Particular fungal morphology acts as a critical index for a successful fermentation. To break the bottleneck of morphological analysis, we have developed a reliable method for fungal morphological analysis. By this method, we can prepare hundreds of pellet samples simultaneously and obtain quantitative morphological information at large scale quickly. This method can largely increase the accuracy and reliability of morphological analysis result. Based on that, the studies of Aspergillus niger morphology under different oxygen supply conditions and shear rate conditions were carried out. As a result, the morphological responding patterns of A.niger morphology to these conditions were quantitatively demonstrated, which laid a solid foundation for the further scale-up.