诱导子对雷公藤不定根生长和次生代谢产物含量的影响

Effects of elicitors on growth of adventitious roots and contents of secondary metabolites in Tripterygium wilfordii Hook. f.

摘要:

为探讨真菌诱导子以及硝酸银等非生物诱导子对雷公藤不定根生长及次生代谢产物含量的影响。以雷公藤不定根为材料，通过向培养基中添加不同种类的真菌诱导子以及硝酸银等非生物诱导子，采用高效液相色谱检测不定根中雷公藤甲素和生物碱含量。结果表明：各种真菌诱导子不影响不定根的生长，但对次生代谢产物含量有显著影响，其中，苹果炭疽和柿子炭疽诱导子的加入不仅使不定根中雷公藤甲素的含量分别提高了2.24和1.93倍，生物碱的含量也各提高了2.02和2.07倍。苹果炭疽诱导子浓度为50 μg/mL时比较适合雷公藤不定根生长及雷公藤甲素和生物碱的积累。硝酸银抑制不定根的生长和生物碱的积累，但促进雷公藤甲素的积累。硝酸银浓度为25 μmol/L时雷公藤甲素含量为对照的1.71倍。茉莉酸甲酯浓度为50 μmol/L时，不定根增长量为对照的1.04倍，雷公藤甲素和生物碱含量分别为对照的1.64倍和2.12倍。酵母提取物浓度为2 g/L时，雷公藤甲素含量为对照的1.48倍。表明培养基中添加硝酸银和酵母提取物对不定根中雷公藤甲素的合成具有明显的促进作用，苹果炭疽和茉莉酸甲酯的协同作用既能促进雷公藤甲素的合成又能促进雷公藤生物碱的合成。

Abstract:

To study the effects of the extract of fungal elicitor, AgNO3, MeJA and yeast on the growth and content of secondary metabolites of adventitious roots in Tripterygium wilfordii. The above elicitors were supplemented to the medium, the growth and the content of secondary metabolites were measured. When the medium was supplemented with the elicitor Glomerella cingulata or Collectotrichum gloeosporioides, the content of triptolide was increased by 2.24 and 1.93-fold, the alkaloids content was increased by 2.02 and 2.07-fold, respectively. The optimal concentration of G. cingulata was 50 μg/mL for accumulation of triptolide, alkaloids and for the growth of adventitious roots. AgNO3 inhibited the growth of adventitious roots and the accumulation of the alkaloids, whereas it (at 25 μmol/L) increased the accumulation of triptolide by 1.71-fold compared to the control. The growth of adventitious roots, the contents of triptolide and alkaloids were increased 1.04, 1.64 and 2.12-folds, respectively when MeJA was at 50 μmol/L. When the concentration of yeast reached 2 g/L, the content of triptolide increased 1.48-folds. This research demonstrated that supplementation of AgNO3 and yeast enhanced the biosynthesis of triptolide in adventitious roots and the synergism of G. cingulata and MeJA could promote the biosynthesis of both triptolide and alkaloids.