异源表达afsRScla全局性调控基因提高工业菌株纳他霉素产量

Improvement of natamycin production in an industrial strain by heterologous expression of afsRScla global regulatory genes

摘要:

前期研究表明棒状链霉菌的全局性调控基因afsRScla异源表达可以激活变铅青链霉菌中两种抗生素的合成。本研究将包含afsRScla基因的质粒pHL851整合到纳他霉素工业生产菌株褐黄孢链霉菌TZ1401的基因组中，使纳他霉素产量提高38%，达到3.56 g/L。qRT-PCR检测6个纳他霉素生物合成基因的转录情况，发现其转录水平提高1.9?2.7倍，表明afsRScla通过正调控纳他霉素生物合成基因的转录，从而提高纳他霉素的产量。本研究结果对afsRScla在抗生素工业生产菌株的高产育种应用具有借鉴意义。

Abstract:

The afsRScla global regulatory genes from Streptomyces clavuligerus activate the production of two antibiotics in Streptomyces lividans. In this study, we gained an increase of 38% in the production of natamycin (3.56 g/L) in an industrial strain Streptomyces gilvosporeus TZ1401 through the integration of pHL851 that bears the afsRScla global regulatory genes into its genome. We discovered by quantitive real-time reverse transcription PCR (qRT-PCR) that the expression of 6 genes of the natamycin biosynthetic gene cluster were improved from 1.9 to 2.7 times. This suggests that afsRScla improve the production of natamycin through increased transcription. This study provides a good example for applying afsRScla in high yield breeding of industrial antibiotic producers.