基于RNA-seq的能源植物芒转录组分析

Transcriptome analysis of bioenergy plant Miscanthus sinensis Anderss by RNA-Seq

摘要:

芒 (Miscanthus sinensis Anderss) 是多年生C4草本植物，可为能量和纤维素产品生产提供高品质的木质纤维素材料，是一种理想的能源植物。采用Illumina HiSeq? 2000 高通量测序技术，对芒花芽和叶芽进行转录组分析。经拼接组装共获得98 326个Unigene，序列平均长度822 bp, N50为1 337 bp。将Unigene 序列与NR、NT、Swiss-Prot、KEGG、GO和COG数据库进行比对 (Evalue<1e-5)，共有74 134 条Unigene 获得了基因注释，占总Unigene的75.40%。其中，通过GO功能分类，45 507个Unigene映射到GO不同的功能节点上；通过KEGG pathways分析，共有36 710个Unigene参与了128个代谢通路；比对到同源序列比例最高的物种分别为高粱 (37 731，60.86%)、玉米 (16 258，26.22%)、水稻 (3 065，4.94%)，共占所有同源序列的92.02%。此外，获得了芒C4关键酶相关基因24个。这些注释信息的完成为芒功能基因及相关候选基因的发掘提供了重要依据。

Abstract:

Miscanthus sinensis Anderss is a perennial C4-grass. It is a promising bioenergy plant, which has been proposed as general feedstock for biomass and lignocellulosic biofuel production. In this study, the flower and leaf buds transcriptomes of Miscanthus sinensis Anderss were sequenced by the platform of Illumina HiSeq? 2000. In total 98 326 Unigenes were generated by de novo assembly with an average length of 822 bp and N50 of 1 023 bp. Based on the NR, NT, Swiss-Prot, KEGG, GO and COG databases (Evalue<1e-5), 74 134 (75.40%) Unigenes were annotated. A total of 45 507 Unigenes were mapped into different GO terms. In KEGG pathways identification, 36 710 sequences were assigned to 128 KEGG pathways. Sorghum bicolor (37 731, 60.86%), Zea mays (16 258, 26.22%), and Oryza sativa (3 065, 4.94%) showed high similarity to Miscanthus sinensis Anderss. And 24 photosynthesis-related enzyme genes were identified. The result provides a foundation for further characterizing the functional genes in Miscanthus sinensis Anderss.