一种非手术性小鼠胚胎移植技术

A nonsurgical embryo transfer technique in mice

摘要:

为了提高非手术性小鼠胚胎移植技术的成功率，利用塑料移植导管模拟非手术胚胎移植过程，通过观察染料在子宫角的分布而评估胚胎移植效果，并在此基础上将自然妊娠3.5 d的小鼠囊胚经子宫颈移植受体小鼠。结果表明：将CD-1小鼠囊胚移植假孕2.5 d小鼠单侧子宫角，平均70.9%的胚胎能够发育至成活新生仔鼠，建立了高效非手术性小鼠胚胎移植技术。该方法简便快捷、不易污染、费用低，无需专业的手术器械，且符合实验动物伦理原则，完全可以取代手术法胚胎移植技术，更重要的是，它为人类和其他大动物的胚胎移植提供了研究模型。

Abstract:

Although efficient nonsurgical transfer of embryos in mice would provide many advantages over a surgical method, the low success rate of nonsurgical transfer has hampered its acceptance and use. Here, a plastic catheter was used to mimic embryo transfer process and then the transfer efficiency was evaluated by intrauterine trypan blue dye dispersion. Also 3.5-day blastocysts from natural pregnant mice were transferred through cervix into uterine horns. The results show that 70.9% of CD-1 mouse 3.5-day blastocysts transferred into unilateral uterine horns of pseudopregnant 2.5-day recipients can be developed to live newborns, and an efficient mouse nonsurgical embryo transfer technique was established. The technique was simple, rapid, inexpensive, unlikely to get contaminated, ethical and do not need specialized apparatus, and can completely replace surgical embryo transfer techniques. Moreover, the mouse nonsurgical embryo transfer technique provides a research model for human and other large animal embryo transfer.