利用表面增强拉曼光谱技术分析温度及pH值对H1N1亚型流感病毒增殖的影响

Effects of temperature and pH on the growth of H1N1 subtype of influenza A virus by surface-enhanced Raman spectroscopy

摘要:

表面增强拉曼光谱 (SERS) 是一种基于纳米颗粒的拉曼光谱，可以高灵敏度地检测流感病毒等重要病原微生物，鉴定不同毒株间的差异。为了建立一种快速检测流感病毒SERS的方法，本实验利用SERS技术对流感病毒H1N1亚型不同毒株在不同温度和pH值的条件下进行了病毒毒价强弱的检测，将流感病毒样品与金纳米颗粒混合静置后用拉曼共聚焦显微镜进行激光扫描。结果显示在pH为7.2、温度为37 ℃的条件下3个H1N1亚型的毒株SERS检测结果显示均出现至少1个大于 (或等于) 3 000的峰值，该状态下病毒毒价最强，最适合病毒生长。另外，细胞生物学方法与SERS技术结果一致，检测中均表现出较好的稳定性和准确性。

Abstract:

Surface enhanced Raman spectroscopy technology (SERS), using gold nanoparticles as a base, was developed for rapid and sensitive detection of virus strains. SERS can be used as a rapid and reliable method to distinguish the titers of viral replication. In the present study, we characterized H1N1 subtypes of influenza A virus strains in different conditions of pH or temperatures, while we analyzed data from SERS technology using gold nanoparticles as a base and cell cultures were employed to further confirm the data from virus strains. Origin8.0 was used to collect Raman spectra, smooth and homogenize data, and to contrast spectra. Our results indicated that the peaks of different virus strains in optimal environmental conditions (T=37 ℃/pH=7.2) reached ≥3 000. This criterion was verified by subsequent virological method. The present data indicate that the established SERS protocol can be used as a rapid and reliable method to distinguish the replication rate of virus, which can be further used in clinical samples.