内含肽在构建亲和纯化系统中的应用

Application of inteins in building protein affinity purification system

摘要:

内含肽是前体未成熟蛋白中的一段具有自我剪接功能的多肽链，在蛋白质纯化、蛋白质连接、环肽制备、蛋白标记以及生物传感器等方面广泛应用。本文综述了内含肽应用于蛋白质亲和纯化的发展历程，分别对层析型和非层析型内含肽纯化体系进行了分析和讨论，并总结了对控制内含肽断裂反应所进行的研究，为进一步改善内含肽介导蛋白质纯化提供依据和线索。

Abstract:

Intein is a part of polypeptide in the premature protein with the capability of self-splicing, which is widely applied in protein purification, protein conjuction, cyclopeptide preparation, protein labeling and biosensor. In this review, we summarized the development of intein used in protein purification, discussed intein-mediated chromatographic and non-chromatographic purification systems, and summarized the researches in manipulating intein cleavage reaction. This work is to provide clues for improvement of intein-mediated protein purification.