基因芯片技术筛选转SlNAC1基因拟南芥差异表达基因

Gene chip analysis of differentially expressed genes in transgenic SlNAC1 Arabidopsis

摘要:

研究已表明植物特有的一些NAC (NAM, ATAF1/2, CUC2) 转录因子可提高植物抗逆性，利用基因芯片技术筛选转SlNAC1基因拟南芥与野生型拟南芥间差异表达基因，能够为研究转基因拟南芥非生物胁迫抗性相关基因提供依据。结果显示，在转SlNAC1基因拟南芥43 604个基因中有3 046个差异表达2倍以上的基因。对差异表达5倍以上基因经过GO富集度统计学分析表明，细胞组分相关基因占33.05%；分子功能相关基因占33.95%；生物学过程相关基因占33.00%。对差异表达2倍以上基因进行KEGG信号通路分析，结果表明有2 431个基因涉及到88个不同的信号通路。通过筛选获得转基因拟南芥非生物胁迫抗性相关候选基因，为后续研究NAC转录因子的下游基因及其调控网络的构建提供方向和理论支撑。

Abstract:

Studies have shown that some plant-specific NAC (NAM, ATAF1/2, CUC2) transcription factors may increase plants resistance to stress. We screened the genes differentially expressed in transgenic SlNAC1 Arabidopsis compared to the wild type by cDNA microarry, to provide scientific basis for studying the genes related to abiotic stress responses in transgenic Arabidopsis. There were 3 046 genes differentially expressed more than twice in the total 43 604 genes of transgenic SlNAC1 Arabidopsis. Gene ontology analysis was used on genes differentially expressed more than five-fold. Genes relevant to cellular components occupied 33.05%, genes correlated with molecular function accounted for 33.95% and genes pertinent to biological process constituted a 33.00% portion. The genes differentially expressed more than twice were processed through kyoto encyclopedia of genes and genomes pathways enrichment (KEGG) analysis. The total 2 431 genes were involved in 88 different signaling pathways. The screened genes related to abiotic stress responses provide direction and theoretical support for the following research on the downstream genes regulated by NAC and construction of the regulatory networks.