三种细胞共培养微流控芯片的设计和制作

Design and fabrication of a microfluidic chip for the co-culture of three cell types

摘要:

设计一种具有“微坝”和“微缝”结构的微流控芯片，能够物理隔离不同细胞，而且培养基中小分子营养物质可以自由流通。实验结果表明在芯片上可以共培养人肺腺癌细胞 (A549)、人胚肺成纤维细胞 (HLF-1) 和人内皮细胞 (HUVECs) 三种细胞，在72 h培养后三种细胞生长状态良好，具有细胞图形化的特点和功能，为下一步开展多种细胞相互作用等相关研究提供重要的技术平台。

Abstract:

Here a microfluidic chip with ‘micro-dam’ and ‘micro-gap’ has been designed and fabricated. It could isolate different cells and flow of medium in each region. It was found that the chip could realize the cells co-culture and patterning of human lung adenocarcinoma cell (A549), human embryonic lung fibroblast (HLF-1) and human endothelial cells (HUVECs), respectively. After 72 hours of culture, three kinds of cells grew well. It provided a developing technical platform for cell related research.