重组蛋白正确折叠与修饰的提高策略

Strategies to improve the folding and modification of recombinant proteins: a review

摘要:

随着分子生物学的研究和不断发展，基因表达技术有了很大的进步。到目前为止，人们已经研究出多种表达系统用以生产重组蛋白，但没有一种表达系统能够完全满足当前需要，各种活性肽和蛋白质类药物的需求逐年攀升，不仅对表达量有要求，更需要正确的翻译后折叠、修饰，使表达蛋白和天然构象更加接近，具有更高的活性和稳定性。结合目前的研究工作从表达系统与宿主、分泌表达、共表达、融合表达和培养条件等方面综述了其对重组蛋白正确折叠以及翻译后修饰的影响，并提出可能改进的策略。

Abstract:

Gene expression technology has made great progress with the continuous developments and researches of molecular biology. Though many systems to produce recombinant proteins have been studied, none of them is available so far to satisfy the needs completely. With the increasing demands of bioactive peptides and protein drugs, expression quantity and correct posttranslational folding and modifications are also needed under the circumstance which can make proteins more close to native conformation and higher activity and stability. Based on our previous work, we summarized the factors affecting the folding and modifications of recombinant proteins correctly from five aspects, including expression system and hosts, secretory expression, coexpression, fusion expression, and the culture conditions, as well as improvement strategies.